HPLC测定冬凌草甲素固态类脂纳米粒在小鼠肝脏中的药物浓度

Determination of oridonin in mice liver following tail intravenous injection of oridonin solid liposome nanoparti-cles by HPLC

目的建立冬凌草甲素固态类脂纳米粒在小鼠肝赃中药物浓度的HPLC 方法小鼠肝脏匀浆液用醋酸乙酯提取,氮气挥干,残渣用甲醇溶解后进样采用Phenomenex-ODS柱(150mm×4.60mm,5μm),以甲醇-水(60:40)为流动相,流速1.0mL·min-1,检测波长为239nm,外标法定量结果冬凌草甲素的线性范围为0.5-90μg·g1(r=0.997 7);最低检测限为20ng; 低、中、高3个浓度的萃取回收率为73.76%-79.08%,方法回收率为85.14%-90.58%,日内精密度≤7.30%,日间精密度≤13.63%,n=5 结论该方法简便、灵敏、准确。

OBJECTIVE: To establish HPLC assay for the determination of oridonin concentration in mice liver. METHODS: The liver samples were extracted with ethyl acetate and the solvent was evaporated to dryness by N2. The residue was dissolved by methanol. The separation was performed on a Phenomenex-ODS column (150 mm × 4.60 mm, 5 μm) with the mobile phase consisted of methanol water (60: 40) at a flow rate of 1.0 mL&middot'min -1. UV detection wavelength was at 239 nm. RESULTS: The calibration curve was linear over the range of 0.5 - 90 μg&middotg-1 (r = 0.997 7)and the limit of detection was 20 ng. The extraction recoveries were 73.76% - 79.08% (n = 5). The recoveries of the assay were 85.14% - 90.58% (n = 5). The RSDs of intra-day and inter-day didn't exceed 7.30% and 13.63% (n = 5), respectively. CONCLUSION: The present study provided a sensitive, accurate and reliable method for the determination of oridonin in mice liver and the evaluation of hepatic targeting efficiency.