固相萃取——紫外导数光谱法测定肝中心得安含量

Determination of Propranolol in Human Liver by Solid-phase Extraction and UV Derivative Spectrophotometry

文章给出了肝中心得安的固相萃取———紫外导数光谱法。肝中心得安用GDX403树脂吸附然后用甲醇洗脱,用二阶紫外导数光谱测定心得安的含量。该法简便快速,方法的回收率和检出限分别为80 6%±3 4%和1 8μg/g。

Determination of propranolol in human liver by solid-phase extraction and UV derivative is presented in this paper. In this method, the propranolol were extracted by GDX403 resin and eluted by methanol. The content of propranolol were obtained by determing UV 2nd derivative spectra of elutent. This method is simple and rapid. The recoveries of propranolol at skiped 10μg/gin liver were 80.6%±3.4%.The detection limits of in liver were 1.8μg/g.