一株嗜热产孢子脱硫肠状菌的系统发育分析

Lineages evolution of a thermophilic spore-forming desulfotomaculum isolated from oil-field

从油田注水联合处理站里分离得到的一株嗜热、耐酸、异养型硫酸盐还原菌,编号为CW01.该菌株兼性厌氧、革兰氏阳性、有极生鞭毛、直或弯曲的短杆状,能产孢子.主要生理生化特性包括生长pH范围为2.8～11,最佳生长pH为5～6.5,最佳生长盐度为1.3%,最佳生长温度37℃,在15～50℃下培养基中均能使测试瓶有黑色沉淀物产生.如果以硫酸盐为电子受体,则可以在乙酸盐、丙酸盐、乳酸盐、丙酮酸盐、丁酸盐、琥珀酸盐、苹果酸盐、延胡索酸盐、戊酸盐、己酸盐、庚酸盐、辛酸盐、癸酸盐、十三烷基酸盐、十五烷基酸盐、棕榈酸盐、十七烷基酸盐、酵母粉和乙醇等电子供体中生长,也能够利用葡萄糖、果糖作为唯一碳源生长.在乳酸盐为电子供体时,能够在硫酸钠、硫代硫酸钠、亚硫酸钠等电子受体中生长.从腐蚀防治的角度对该菌株的腐蚀速率、耐1227性质以及生长曲线也进行了测定.通过16srDNA测序(GenBank上的AC号为AY742958)以及系列比对分析该菌属于细菌界(Bacteria)、厚壁菌门(Firmicutes)、梭菌纲(Clostridia)、梭菌目(Clostridiales)、蛋白胨链球菌科(Peptococcaceae)、脱硫肠状菌属(Desulfotomaculum),与该属中的种Desulfotomaculumputei相似性达到98%.

A novel thermophillic, acidityphilic and heterophic sulfate-reducing bacteria, strain numbered CW-01 was isolated from the united management stage in oil-field, which was an facultative anaerobe, non-motile. Gram-positive, pole flagellum, spore-forming curved rod growing within a pH range of 2.8～11 (optimal growth at pH5～6.5) and the temperature of 15～50?℃ (optimal growth at 37?℃). While sulfate and sulfite were used as electron acceptors, a limited number of compounds were utilized as electron donors: acetate, lactate, pyruvate, butyrate, succinate, malate, fumarate, valerate, caproate, heptanoate, octanoate, decanoate, tridecanoate, pentadecanoate, palmitate, heptadecanoate or ethanol, yeast extract and glucose, fructose as only sources of carbon. When lactate was used as electron donors, sulfite and thiosulfate could be used as electron acceptors. The growth curve of SRB, anti-chemical of 1?227 and corrupted velocity were measured. The sequence slowed strain CW-01 (accession numbers: AY742958) was classified as a representative of a new strain of the genus desulfotomaculum, desulfotomaculum putei (Bacteria, Firmicutes, Clostridia, Clostridiales, Peptococcaceae) whose similarity is 98?%.