Investigation of molecular mechanism of CNS symptoms induced by DDVP poisoning

Objective To Screen differentially expressed genes related to dichlorphos(DDVP) poisoning from rat's hippocampus using oligonucleotide microarray technology in order to elucidate the mechanism of DDVP poisoning. Methods We composed probes of 40 genes of our interest. The probes were retrotranscribed on plata glass and oligonucleotide microarray was formed. 0.5 ml DDVP was given to the rats in experimental group and 0.Sml-pumping brine was given to the rats in control group by hypodermic injection, twenty minutes after convulsion, all hippocampus were collected for total RNA extraction, cDNAs were marked with Cy3 and Cy5 for control group and experiment group respectively, and hybridized with loligonucleotide microarray. Hybridization signals were collected and analyzed following scanning by laser co-focal scanner. Results There were 8 differentially expressed genes identified. Conclusion Many genes expressing changed by DDVP poisoning could be analyzed in a time period by using oligonucleotide microarray, which provides a powerful method for further studies on the molecular mechanism of DDVP poisoning.