一氧化碳对高血压大鼠阻力动脉重塑的影响

Influence of carbon monoxide on resistant artery remodeling in hypertensive rats

目的 探讨一氧化碳(CO)对高血压大鼠阻力动脉重塑的影响。方法 32只Wistar大鼠随机分为对照组(A组)、高血压组(B组)、Hm干预组(C组)和Hm+HLL(D组)干预组。10周时测CO、NO、ET-1、AngⅡ浓度;比较各组肠系膜动脉三级分支的结构变化。结果 B组血压升高,C、D组较B组降低。C、D组血浆CO较A、B组升高,D组高于C组;B、C、D组血清NO较A组降低,D组较B、C组升高;B、C组血浆ET-1较A、D组升高;血浆AngⅡ四组间无统计学差异。B组发生管腔重塑,C、D组较B组改善。结论 CO能防止高血压阻力动脉重塑;其机制可能与CO降低血压、升高NO浓度及降低ET 1浓度有关。

Objective To investigate the influence of carbon monoxide on resistant artery remodeling in hypertensive rats. Methods 32 Wistar rats were randomly divided into control group(A), hypertensive group(B), Hm interventional group(C) and Hm+HLL interventional group(D). Blood pressure was measured weekly. The concentration of CO,NO, Ang Ⅱ and ET-1 and the expression of heme oxygenase-1 protein was measured at 10th week. The structure of mensenteric artery 3rd branch was compared among the four groups. Results Blood pressure increased in group B, and was lower in group C and D than in group B(P<0.01). The concentration of CO in group C and D were higher than that of group A and B(P<0.001),and it was higher in group D than in group C(P<0.001). The concentration of NO in group B, C and D were lower than that of group A(P<0.001), and it was higher in group D than in group B and C(P<0.001). The concentration of ET-1 increased more in group B and C than in group A and D(P<0.05). There were no significant differences of Ang Ⅱ concentration among the four groups. Mensenteric artery remodeling took place in group B, and it was ameliorated in groups C and D compared to group B(P<0.001). Conclusion Carbon monoxide can prevent hypertensive rats from mensenteric artery remodeling. The mechanisms may be that carbon monoxide decrease blood pressure and the concentration of ET-1 increase the concentration of NO.