摘要
目的:观察当归多糖及其3个组分对淋巴细胞增殖和细胞因子IL-2、IFN-γ的影响.方法:机械分散法制备小鼠单个脾细胞悬液;MTT法检测细胞增殖;酶联免疫法测定培养上清液中IL-2和IFN-γ的浓度.结果:当归多糖及其3个组分在30~100 μg/ml剂量范围内,能显著促进脾细胞的增殖;其中组分AP-3能够显著促进巨噬细胞、混合淋巴细胞的增殖反应,剂量依赖性地增加细胞上清液中IL-2和IFN-γ的浓度.结论:当归多糖能够活化巨噬细胞和T细胞,还可以通过细胞因子IL-2、IFN ̄γ发挥免疫调节作用.
Objective: To investigate the effects of Angelica sinensis polysaccharide(AP) and its three fractions(AP-1,AP-2 and AP-3) on lymphocyte proliferation induction of IL-2 and IFN-γ. Methods: Effects of AP on proliferation of murine splenocyte, macrophage, mixed lymphocyte reaction were tested by MTT assay. The production of IL-2 and IFN-γ in supernatant were determined by ELISA. Results: At the range of 30~300 μg/ml, AP and its three fractions directly stimulated proliferation of murine splenocyte; AP-3 significantly enhanced the proliferation of macrophage and mixed lymphocyte, the treatment of AP-3 augmented the production of IL-2 and IFN-γ in cell culture supernatant. Conclusion: AP could active macrophage and T lymphocyte, also regulate the immune response through upregulating IL-2, IFN-γ expression.
出处
《中药材》
CAS
CSCD
北大核心
2005年第5期405-407,共3页
Journal of Chinese Medicinal Materials