摘要
生理盐水配制的木耳单核担孢子悬浮液，用剂量１００－２０００Ｇｙ、剂量率２０Ｇｙ／ｍｉｎ的钻６０ｖ射线辐照后，挑取３１００个辐照处理单孢萌发菌落在完全培养基和基础培养基上筛选，共获得９株稳定的营养缺陷型单核菌株。辐照诱变频率２．３８×１￣（－３）－４．４４×１０￣（－２）。营养缺陷型菌株原生质体回复突变率小于１０￣（－６）。营养缺陷类型包括氨基酸、维生素和核酸碱基的单缺类型和复合多缺类型。
suspension of monokaryotic basidiospores of Auricularia auricula and A.fuscosucinea were radiated with ￣(60)Co gamma ray.The dosage employed was 100-2000 Gy,With dosage rate 20 Gy/min.,After radiation,3100 radiated monospore colonies were picked out. Then they were transfered onto tbe comolete midium(CYM) and also the minium midium (MM).Through such screening 9stable auxotrophic monokaryotic mutant Strains were obtained.The results revealed that the radiational mutagenic frequency was 2.38 ×10￣(-3)-4.44 × 10￣(-2).The reverse mutation frequency of the protoplast of the auxotrophic mutants was less than 10￣(-6). The types of the auxotrophic mutants included mono-defect type & poly-defect type of either amino acid or vitamin or nucleic acid base.
出处
《中国食用菌》
1994年第2期19-21,共3页
Edible Fungi of China
关键词
木耳
营养缺陷型
钴60V射线
Auricularia
Auxotrophic mutant
￣(60)Co gamma ray
