银耳菌分离方法的研究

Study on the isolation of mycelia of Tremella fuciformis

用单孢分离、基本分离和组织分离获得银耳菌，银耳菌的纯培养与伴生菌长在一起成为银耳母种。在三种方法中，以单孢分离获得的银耳原基、基内菌丝与伴生菌配制的母种质量好，成功率亦高。基内分离纯培养的方法不如单孢分离的方法好，基内分离银耳菌丝和伴生菌的混合物，得到的母种质量不稳定。组织分离的方法不能采用。

The mycelia of Tremella fuciformis can be obtained by single spore isolation, mycelia isolation in medium or tissue isolation . These pure cultures can be cultured with associated fungi and thus become the master cultures of T. fuciformis. Among the three methods,the single spore isolation is the best one,Because primordia and medium mycelia,which are got by single spore isolation,can produce the master cultures with associatedfungi, the quality of the master culture is good and also can be got easily.But the pure culture of mycelia isolation in medium is not as good as that of singlespore isolation.The.mixed matter of the mycelia and the associated fungi which are obtained bymycelia isolation in medium is not sable. The way of tissue isolation is not practical.