摘要
猴头菌点种法单孢分离是在注射法和稀释法基础上改进的一种单孢分离方法。经７５２次单孢分离，已成功获得１４６个单核菌丝。单孢率为４４．４％，双孢率不足１％，零孢率为５４．６％。实验证明点种法单孢分离可靠，且点种器制作简单，取材方便成本低，是目前较适宜普及推广应用的单孢分离方法。
Based on the injection method and the dilution method,the drop culture method(DCM)was designed for the monospore isolation of Hericium erinaceus of 752 drops,onespore drops was 44.4%, two spore drops less than 1%and spore-free drops 54.0%,Theresults showed the DCM was effective and practicable, and the instrument for the DCM was simple and inexpensive.
出处
《中国食用菌》
1994年第5期7-8,共2页
Edible Fungi of China
关键词
猴头菌
点种法
单孢分离
Drop culture method,Monospore isolation,Hericium erinaceus
