抗人膀胱癌/抗VEGF双功能基因抗体的动物实验研究

Study on animal experiment of anti-human bladder carcinoma/anti-VEGF bispecific gene antibody

目的探讨抗人膀胱癌/抗VEGF双功能基因抗体对人膀胱癌生长及淋巴转移的影响。方法通过构建人膀胱癌裸鼠皮下移植瘤模型并注射双功能抗体,观察肿瘤生长及盆腔淋巴结转移情况,同时采用免疫组化法检测肿瘤微血管密度值及凋亡的肿瘤细胞指数。结果肿瘤大小实验组为(19.50±4.51),对照组为(57.62±8.31),两组比较差异有极显著性意义(P<0.01);肿瘤微血管密度实验组为(2351±207),对照组为(4356±548),两组相比差异具有显著性意义(P<0.05);凋亡指数实验组为19.25,对照组为9.31,两者比较,差异有统计学意义(P<0.05);盆腔转移实验组无一只发生,而对照组为75.0%,两组比较,差异有极显著性意义(P<0.01)。结论抗人膀胱癌/抗VEGF双功能基因抗体对人膀胱癌具有良好的靶向性,能够通过抑制肿瘤微血管形成和加速肿瘤细胞凋亡,遏制实验性人膀胱癌的生长转移,为该抗体用于临床膀胱癌的治疗提供了一定的实验依据。

Objective To explore the effect of bispecific gene antibody (anti-human bladder carcinoma/anti-VEGF) on growth and lymphatic metastasis of human bladder carcinoma. Methods Bispecific antibody was injected into nude mice nearby xenograft tumor of human bladder transitional cell carcinoma to observe the growth and lymphatic metastasis in pelvic cavity of carcinoma. The carcinoma capillaries density value and apoptotic carcinoma cellular indexes were determined by immunohistochemistry.Results There was significant difference in tumor size, capillaries density and apoptotic index between experimental group〔(19.50±4.51), (2 351±207) and 19.25〕 and control group 〔(57.62±8.31), (4 356±548) and 9.31〕 (P<0.01, 0.05, 0.05, repectively). There was no no pelvic metastasis in experiment group, while metastasis rate of control group was 75.0% (P<0.01).Conclusions Bispecific gene antibody with better target orientation could suppress xenograft tumor growth of human bladder transitional cell carcinoma by antiangiogenesis and apoptosis, which provide certain experimental basis for this antibody used in clinical treatment of bladder carcinoma.