血瘀证患者血小板CD62p基因、白细胞HSP70基因的表达

Expression of Platelet CD62p Gene and Leucocyte HSP 70 Gene in Patients with Blood Stasis Syndrome

目的从基因水平探讨血瘀证微观实质。方法以病证结合方式,严格按照诊断标准选取16 0例高血压病和2型糖尿病血瘀证(10 0例)和非血瘀证患者(6 0例) ,用血液生化指标和实验技术,进行血小板CD6 2 p基因、白细胞HSP70基因的表达的观察、对比、分析,并以健康人为对照。结果血瘀证患者存在CD6 2 p基因、HSP70基因的异常表达,两者表达水平较健康人显著升高(P <0. 0 1) ,且基因异常表达水平的高低与血瘀证各型之间存在密切关系。结论CD6 2p基因和HSP70基因为与血瘀证有密切相关的基因。

ObjectiveTo investigate microcosmic essentials of blood stasis syndrome (BSS) at the gene level. MethodsOne hundred and sixty patients with hypertension or diabetes mellitus, BSS or non-BSS, were strictly selected in disease-syndrome integrated mode according to diagnosis standard. The expression of CD62p gene and HSP 70 gene in them were observed, compared and analyzed with blood biochemical indexes by experimental techniques, and compared to those in healthy subjects as control, for exploring the microcosmic mechanism and evolutive rules of BSS formation at the gene level. ResultsIt was showed, through determination and analysis of the microcosmic indexes closely associated with BSS, that the expression of CD62p gene and HSP70 gene were abnormal in patients with BSS, they were increased in patients with BBS and significantly higher than those in healthy subjects (P<0.01). The abnormality and level of them were closely correlated with the types of BSS. ConclusionCD62p gene and HSP70 gene might be closely associated with BSS.