四君子汤对小鼠膀胱癌化疗的减毒增效作用

Toxicity Attenuation and Efficacy Potentiation Effects of Sijunzi Decoction on Bladder Carcinoma Treated by Chemotherapy in Mice

目的探讨中药四君子汤对小鼠膀胱癌化疗的减毒增效作用。方法采用小鼠膀胱癌细胞接种于T 739小鼠皮下,动物随机分为8组:对照组,丝裂霉素C(mitomycinC ,MMC)常规剂量组,MMC小剂量组,四君子汤大剂量组,四君子汤中剂量组,四君子汤小剂量组,联合用药(四君子汤大剂量+MMC常规剂量)A组,联合用药(四君子汤大剂量+MMC小剂量)B组;每组于接种癌细胞2 4h后分别给药;用药14天后分别检测各组小鼠的抑瘤率、腹腔巨噬细胞活性,观察小鼠荷瘤生存时间及治疗7天后血白细胞数的变化。结果四君子汤大剂量组的荷瘤小鼠生存时间与对照组比较差异有显著性(P <0 .0 5 ) ,而四君子汤中、小剂量组与对照组比较差异无显著性(P >0. 0 5 ) ;MMC常规组的抑瘤率最高,但荷瘤生存时间与对照组比较差异无显著性(P >0 . 0 5 ) ;联合用药B组的小鼠荷瘤生存时间最长,达到(32 7±1 3)天,与其他各组比较差异均有显著性(P <0 . 0 5 ) ;联合用药B组除抑瘤率低于MMC常规组及联合用药A组,血白细胞数及巨噬细胞活性均优于MMC各剂量组及联合用药A组,差异均有显著性(P <0 . 0 5 )。结论四君子汤联合小剂量MMC化疗可以有效抑制小鼠膀胱肿瘤生长,具有增效减毒之作用。

ObjectiveTo investigate the toxicity attenuation and efficacy potentiation effects of Sijunzi decoction (SJZD) on bladder carcinoma treated by chemotherapy in mice. MethodsT739 mice were randomly divided into 8 groups after subcutaneous inoculation of bladder carcinoma cells, the control group (A); two mitomycin C (MMC) group, treated with MMC of routine dosage (B) and low-dosage (C) respectively; three SJZD groups, treated with SJZD of high (D), medium (E) and low-dosage (F) respectively; and two combined treatment groups, treated with SJZD of high-dosage + MMC of routine dosage(G) and SJZD of high-dosage + MMC of low-dosage(H). The medication was begun at 24 hrs after inoculation. The tumor inhibitory rate, activity of peritoneal macrophages after 14 days of treatment and change of peripheral white blood cells after 7 days of treatment were determined and the survival time of mice was observed. ResultsThe survival time of mice in Group D was significantly higher than that in Group A (P<0.05), while those in Group E and F showed insignificant difference as compared with those in Group A (P>0.05). The highest tumor inhibitory rate was shown in Group B, but the survival time in that group showed no significant difference as compared to those in Group A (P>0.05). The longest survival time (32.7±1.3 days) was shown in Group H, which was obviously different to that in other groups (P<0.05). And the leukocyte counts and macrophage activity in Group H were better than those in Group B, C and G (P<0.05), except that the tumor inhibitory rate was significantly lower than that in Group B, C and G (P<0.05). ConclusionCombined chemotherapy of SJZD with low dosage MMC has definite effect in inhibiting tumor growth in mice with bladder carcinoma, displaying special effects of toxicity attenuation and efficacy potentiation.