三七对恒河猴子宫内膜t-pA及PAI-1mRNA表达的影响

Influences of two kinds of active components from panax notoginseng on t-pA and PAI-1 in inflammatory Metrorrhagia model of Macaca mulatta

目的:探讨t-pA及PAI-1在炎性子宫出血中的作用及三七复合有效成分的影响。方法:选择月经正常雌性育龄恒河猴,随机分为中药组(三七复合有效成分)、模型组及正常对照组。运用原位杂交法检测细菌感染性子宫出血模型组、三七复合有效成分中药组及正常对照组恒河猴子宫内膜组织中PAI-1mRNA的表达;运用发色底物法检测三组内膜组织中t-pA的活性。结果:三七复合有效成分组子宫内膜间质、子宫血管内皮细胞及平滑肌细胞胞浆中PAI-1mRNA的表达明显高于模型组,差异有显著性(P<0.05),与正常组相比没有差别(P>0.05);三七复合有效成分组与模型组比较,t-pA活性明显降低,与正常组比较无显著性差别(P>0.05)。结论:炎症因素导致的子宫出血与t-pA活性增高有关,三七复合有效成分减少炎性子宫出血的作用可能与其调节子宫内膜局部t-pA和PAI-1的平衡有关。

Objective:To explore the role of t-pA|PAI-1 in inflammatory Metrorrhagia and the influence of two kinds of active components on them.Methods:Eleven mormal menorrhea adult Rhesus monkeys are choosed as research object,which are divided into four model groups,four TCM group and three Control group at random.The hybridization in situ was performed to estimate the express of PAI-1mRNA in the endometrium of model group,TCM group and normal control group,wiile spectrophotometric assay was performed to estimate the t-pA activity in three groups.Result:The PAI-1mRNA expression in endometrum of TCM group was higher than that of model group (P< 0.05),and was of no difference with normal group.The t-pA activity in endometrum of TCM group was significantly decreased than of model group,and was of no difference with normal group.Conclusion:The increase of t-pA activity may have relation to inflammatory Metrorrhagia,and two kinds of active components from panax notoginseng reduce in flammatory Metrorrhagia by regulating balance of t-pA and PAI-1.