用RAPD分析辣椒细胞质雄性不育基因

RAPD ANALYSIS ASSOCIATED WITH CYTOPLASM MALE STERILITY IN HOT PEPPER(Capsicum annuum L.)

根据近等基因系分析原理 ,以辣椒细胞质雄性不育系 93 A及其保持系 93 B为材料 ,对辣椒细胞质雄性不育基因及其保持基因开展了RAPD标记研究 ,结果表明 :OPK 1 7550 、OPK 1 7150 0 标记可能与细胞质雄性不育基因相连锁 ,OPH 1 1 2 0 0 0 标记可能与保持系中的保持基因相连锁。对侯选标记OPK 1 7550 进行了克隆和部分序列分析 ,为利用混合群体分离法 (BSA)对分离群体中的不同单株进行SCAR分析的标记验证工作奠定了基础。

According to the isogenic line analysis theory, RAPD analysis was undertaken in the cytoplasm male sterile (CMS) line 93-A and its maintainer line 93-B in hot pepper (Capsicum annuum L.). The results showed that the candidate marker OPK-17 550 and OPK-17 1500 were possibly correlated with the CMS gene in the line 93-A, and the candidate marker OPH-11 2000 was possibly associated with the maintainer gene in the line 93-B. The marker OPH-17 550 was cloned and partially sequenced, which provided a basis of further marker verification by SCAR marker and bulked segregation analysis.