经保存的大鼠胚胎后肾同种腹腔内移植模型的建立

Allograft of developing metanephroi into omenta of cyclosporine-treated adult rats after preservation

目的建立胚胎后肾大网膜内移植大鼠模型,并探讨其在受者体内生长、发育情况。方法取孕16d(E16)和17d(E17)的SD大鼠胚胎,切取胚胎后肾,以组氨酸色氨酸酮戊二酸盐液(HTK液)保存3d,然后移植到切除单侧肾脏的成年SD大鼠的大网膜内,另设未经保存的E16胚胎后肾直接移植对照组。术后给予环孢素A皮下注射,术后3～4周后开腹观察器官形成情况;术后8周,切除受者自体肾脏,观察移植后肾的组织学形态,测定后肾功能。结果移植后3周,移植后肾肾单位、集合管及输尿管的结构正常,组织中少有淋巴细胞浸润,电镜显示移植后肾发育的肾血管球细胞及基底膜、近端肾小管、远端肾小管和集合管上皮细胞超微结构正常。移植后8周,移植后肾的湿重、体积、分泌尿量及内生肌酐清除率与对照组比较,差异无统计学意义(P>0.05)。结论E16、E17胚胎后肾大网膜内移植,并辅以环孢素A皮下注射,可以形成器官,并发挥功能。

Objective To evaluate the animal models and the effect on organogenesis and function of embryonic metanephroi allografted into cyclosporine-treated adult rats after their preservation in histidine-tryptophan-ketoglutarate (HTK) solution.Methods Whole metanephroi preserved in HTK for 3 days from embryonic day 16 and 17 (E16, E17) of Sprague-Dawley (SD) rats were grouped and allografted into the omenta of cyclosporine-treated SD adult rats which had one kidney resected. E16 metanephroi implanted directly into omenta were used as controls. 3 to 4 weeks after implantation, metanephroi allografted in host rats were removed for histopathological and electronic microscopy (EM) examination or anastomosed for renal function measurement 8 weeks later.Results 3 to 4 weeks post-implantation, E16 and E17 metanephroi had formed mature nephrons and collecting ducts with few lymphocytic infiltration. EM examination showed they had cellular ultrastructure of normal nephrons and collecting ducts. E16 and E17 metanephroi allografted after preservation for 3 days had no significantly different values of wet weight (P=(0.346),P=(0.705)), urine volume (P=(0.396),P=(0.687)). and creatinine clearances (P=(0.469),P=(0.543)) from those of E16 metanephroi implanted directly.Conclusion E16 and E17 metanephroi allografted into cyclosporine-treated adult rats undergo growth and differentiation and exhibit excretory function after preservation in HTK solution for 3 days.