胶原蛋白膜作为金葡液载体修复骨缺损的实验研究

EXPERIMENTAL STUDY OF REPAIRING BONE DEFECT BY STAPHYLOCOCCUS AUREUS INJECTION CARRIED IN COLLAGEN MEMBRANE

目的 验证胶原蛋白膜吸附金葡液后修复骨缺损的优越性。 方法 选用成年新西兰大白兔2 4只,随机分为2组,每组12只。手术造成桡骨标准缺损后,分别植入胶原蛋白-金葡液复合体(实验组)和单纯等量金葡液(对照组) ,于术后2、4、6及8周时行大体、X线片、组织学及免疫组织化学观察。 结果 整个过程中实验组缺损区新生骨组织增殖明显、持续时间较长,且无过量结缔组织生长;X线片可见连续性骨痂通过缺损区,分布均匀;组织学观察可见多个成骨中心,骨小梁排列有序,成熟骨替代完全;免疫组织化学染色可见骨形成蛋白分布持续时间长,而且在新骨组织中占据范围大。对照组缺损区新生骨组织在质量和数量上都较实验组差。 结论 胶原蛋白能够阻挡周围软组织进入缺损区,为新骨生长提供空间;同时作为金葡液的载体,能减少金葡液外溢。

Objective To validate the advantage of repairing bone defect by staphylococcus aureus injection carried in collagen membrane. Methods Twenty four adult New Zealand rabbits were divided into two groups randomly. After the experimental model of standard bone defect had been made by operation, collagen membrane staphylococcus aureus injection and staphylococcus aureus injection with the same quantity were transplanted in bone defect areas of the two groups respectively. The reconstructed tissues were observed by general method, X ray, histology, and immunohistochemistry at 2nd、4th、6th、8th week respectively. Results The experimental group showed that new bone proliferated distinctly in bone defect area and the proliferation lasted long, and no excessive connective tissue in defect area. X ray observation showed that there was continual callus growth in transplantation area in early stage and the distribution of new bones was even in the group. Histological observation showed that there were many new bone growth centers in bone defect area, trabecular bones were sequentially distributed, and mature bone replacement was complete. Immunohistochemical examination showed that bone morphogenetic protein (BMP) could be seen for a long time and BMP took up a large part in the new bone tissues. Conclusion Collagen membrane could prevent parenchyma from penetrating into bone defect area and provide room for new bone growth. As the carrier of staphylococcus, collagen membrane could reduce the overflow of staphylococcus and improve its curative effect as well.