热休克蛋白27基因在食管鳞状上皮癌变过程中的表达

Expression of heat shock protein 27 mRNA in the carcinogenesis of esophageal squamous epithelium

目的:研究食管鳞癌、癌旁及正常食管粘膜中热休克蛋白27(HSP27)基因的表达。方法:采用RTPCR方法,定量检测26例食管鳞癌、癌旁及正常食管粘膜中HSP27基因表达水平。结果:以HSP27/GAPDHmRNA灰度比值表示HSP27基因的相对表达量。食管鳞癌、癌旁(不典型增生)和正常食管粘膜中的HSP27/GAPDH灰度比值分别为0.81±0.27、0.90±0.24和1.22±0.39。HSP27基因表达水平在食管鳞癌和不典型增生食管粘膜中的表达量明显低于正常食管粘膜(P<0.05),而HSP27基因在食管鳞癌和不典型增生食管粘膜中的表达量差别无显著性(P>0.05)。食管鳞癌、不典型增生食管粘膜及正常食管粘膜中的HSP27基因表达在不同患者年龄、性别和淋巴结转移之间均无显著性差异(P>0.05)。结论:HSP27基因在食管鳞癌和不典型增生食管粘膜中表达与正常食管粘膜相比明显降低,提示HSP27基因在食管鳞癌的发生、发展过程中有不同程度的表达缺失。因此,提高HSP27基因在食管鳞癌中的表达将来可能会成为一种非常有效的生物学治疗手段。

Purpose:To study the expression of heat shock protein (HSP) 27 mRNA in esophageal squamous carcinoma, mucosa adjacent to cancer and normal esophageal mucosa. Methods:The expression of HSP27 mRNA in esophageal squamous carcinoma, mucosa adjacent to cancer and normal esophageal mucosa of 26 cases was quantitatively detected by RT-PCR. Results:The ratios of HSP27/GAPDH in esophageal squamous carcinoma, mucosa adjacent to cancer (atypical hyperplasia) and normal esophageal mucosa were 0.81±0.27, 0.90±0.24 and 1.22±0.39, respectively.The results suggested that the expressions of HSP27 mRNA in esophageal squamous carcinoma and mucosa with atypical hyperplasia were lower than that in normal esophageal mucosa (P<0.05). However, the difference between the expression of HSP27 mRNA in esophageal squamous carcinoma and mucosa with atypical hyperplasia was not significant (P>0.05). There was no obvious difference in the expressions of HSP27 mRNA as to age, gender and metastasis of lymph node (P>0.05). Conclusions:Compared with normal esophageal mucosa, the expression of HSP27 mRNA in esophageal squamous carcinoma and mucosa with atypical hyperplasia was markedly decreased.This indicated that the expression of HSP27 mRNA to a greater or less extent lost in the carcinogenesis and development of esophageal squamous carcinoma. To up-regulate the expression of HSP27 mRNA in esophageal squamous carcinoma may be a very effective biological therapy.