应用组织微阵列技术研究肺癌组织中IGF-Ⅱ的表达

Research on IGF-Ⅱprotein expression in lung cancer by tissue microarray

目的:构建高通量组织微阵列(tissuemicroarray)/组织芯片(tissuechip),检测其上IGFⅡ的表达情况,以明确IGFⅡ表达与肺癌的相关性。方法:用组织阵列仪制备按不同组织学类型排布的组织芯片,然后用免疫组织化学SP法检测一张组织芯片上54例肺癌肿瘤组织和10例正常肺组织样本中的IGFⅡ表达情况,并分析其表达与各临床病理学参数之间的相关性。结果:IGFⅡ蛋白在实验组中的阳性表达率为42.6%(23/54),对照组为阴性,两者之间具有显著性差异(P<0.05);IGFⅡ表达阳性率与大体类型、组织学分级、临床分期有关(P<0.05),而与性别、年龄、组织学分型及淋巴结转移无关(P>0.05)。结论:IGFⅡ的表达与肺癌的恶性行为有关,检测此指标对预测肺癌的预后和指导治疗有一定的参考价值。组织芯片是一较高的技术平台,可以应用此技术进行大规模组织样本的检测。

Purpose:Constructing a high-flux tissue microarray/tissue chip and detecting IGF-Ⅱprotein expression of cases by it, and to determine the correlation between IGF-Ⅱprotein expression and lung cancer. Methods:A series of tissue chips were prepared by using tissue arrayer with samples from lung cancers of different histological classifications. Specimens from 54 cases of lung cancer and 10 cases of normal lung tissues were detected immunohistochemically on a tissue chip for IGF-Ⅱprotein expression and its correlation to clinic-pathological parameters was analyzed statistically. Results:Positive rate of IGF-Ⅱprotein in lung cancer was 42.6%(23/54), which was higher than that of normal lung(0.0%, 0/10, P<0.01); the positive rate of IGF-Ⅱprotein correlated strongly to the gross types, histological differentiations, and clinical stage of lung cancer (P<0.05), while it had no significant relationship to sex, age, histological classifications and lymphoid metastasis(P>0.05). Conclusions:IGF-Ⅱprotein might be related to the malignant behaviors of lung cancer. Detecting the expression of IGF-Ⅱ protein probably can predict the prognosis of lung cancer. It is feasible to utilize tissue chip for screening of clinical tissue specimens on a large scale.