N-Methyl-D-Aspartate受体拮抗剂对脑缺血后成年大鼠神经干细胞内源性激活的实验研究

The investigation of internal activation NSC by NMDA receptor antagonist administration following focal ischemic insults

目的 研究N - Methyl- D- Aspartate(NMDA)受体拮抗剂MK- 80 1对脑缺血后神经干细胞(NSC)激活的作用。方法 将4 0只SD大鼠分成对照组和实验组,两组大鼠均采用传统线栓法作成大脑中动脉缺血再灌注模型,实验组大鼠腹腔注射MK- 80 1,对照组腹腔注射生理盐水,通过免疫组织化学技术标记鼠脑海马齿状回颗粒细胞层(SGZ)、室管膜下层(SVZ)及梗死皮质周边区注射后第3、7、11、18天的Brdu、Nestin阳性细胞数。结果 对照组大鼠Brdu、Nestin阳性细胞7d在SGZ出现一小高峰,然后迅速下降,11d阳性细胞甚少,梗死皮质周边区更少;而实验组Brdu、Nestin阳性细胞3d在SVZ明显表达,7～11d在SGZ区达高峰,并可持续至18d,同样梗死皮质区Brdu、Nestin阳性细胞7～18d表达明显,两组比较,有统计学意义(P<0 .0 1)。结论 NMDA受体拮抗剂MK- 80 1在脑缺血后,能促进NSC的增殖、分化。

objective The effect was investigated to induce NSC proliferation and differentiation in the adult rat SGZ、SVZ and cortex by NMDA receptor antagonist administration following focal ischemic insults produced by middle cerebral artery occlusion (MCAO).Method Forty SD rats are assinged two groups:experiment group (I),control group (II).All rats were subjected to 2h of MACO.Rats of group (I) were intraperitoneally injected MK-801 which is the NMDA receptor antagonist and rats of group(II) were intraperitoneally infected saline.The rate of Brdu postive cells and Nestin postive cells were compared in SGZ,SVZ and infract contex.Result Group(II):Brdu postive cells and Nestin postive cells increased in SVZ at 3 days after reperfusion, reached slightly peaked at 7、11 days in SGZ, decreased after 11 days and scarely detected after 18 days.But in gorup(I):Brdu positive cells and Nestin postive cells significally increased than that in group(II)after 3,7,and 11 days in SVZ and SGZ.The proliferation is found increasing long-lasting after 18 days.Brdu、Nestin postive cells singnifically expressed near infract contex.Conclusion NMDA receptor antagonist treatment serves as a valuable tool to activate NSC proliferation and differentiation in cerebral after infract.