心力衰竭患者心肌组织β_1、β_2、β_3受体mRNA表达改变与心功能的关系

The relationship between expressions of β_1-,β2-,β _3-adrenoceptor mRNA of myocardium and cardiac function in patients with heart failure

目的 探讨不同程度心力衰竭(心衰)患者心肌组织β1、β2、β3 受体mRNA表达的变化及其与心功能的关系。方法 应用逆转录聚合酶链反应方法检测24例瓣膜置换术的心衰患者和5例健康对照者心肌组织β1、β2、β3 受体mRNA表达。结果 心衰时β1 受体mRNA表达减少,心衰程度越重表达越少;β2 受体表达在不同程度心衰患者之间改变差异无统计学意义;β3 受体表达增多,心衰程度越重表达越多。结论 心衰时β1 受体下调,而β3 受体上调,心肌组织β受体mRNA的变化与心功能密切相关。

Objective To investigate the alteration of expressions of β 1-,β 2-,β 3-adrenoceptor mRNA in human myocardial tissue and the relation between their expressions and cardiac function in patient with heart failure. Methods The mRNA expressions of β 1-,β 2- and β 3-adrenergic receptors in myocardial tissue were analyzed by using the reverse transcriptase-polymerase chain reaction in 24 patients with heart failure of valvular heart disease and 5 control subjects.Results β 1-adrenergic receptor mRNA expressions in myocardium were significantly lower in patients with heart failure than those in control subjects, and progressively reduced with aggravation of heart function. By contrast, β 3-adrenoceptor mRNA expressions were significantly higher in patients with heart failure than those in controls, and progressively elevated with aggravation of cardiac function. No difference was observed in β 2-adrenergic receptor among all groups.Conclusion The changes of β-adrenergic receptor mRNA expression are associated with the severity of heart failure.