摘要
目的比较不同胃癌细胞系尿激酶型纤溶酶原激活物(uPA)表达及其活性与腹膜转移潜能的关系。方法采用ELISA和Western印迹方法,比较不同胃癌细胞系uPA表达的差异,并测定其活性。在24孔培养板或Boyden小室中与生长良好的间皮细胞共同培养不同时间,在显微镜下直接计数与间皮细胞黏附的胃癌细胞,而用MTT法评估胃癌细胞在间皮细胞间的迁移和侵袭情况。结果4株胃癌细胞(AGS、SGC7901、MKN45和MKN28)的uPA表达以SGC7901最高,uPA活性以MKN45最高,AGS两者皆最低。MKN45的黏附能力明显强于MKN28(P<0.05)、SGC7901(P<0.05)和AGS(P<0.01),但其迁移和侵袭能力与SGC7901和MKN28相比差异无统计学意义;而AGS在3方面均明显弱于其他3株细胞。结论4株胃癌细胞uPA表达量及其活性差异较大,并且与其腹膜转移潜能呈正相关。
Objective To compare the expression and activities of urokinase t ype plasminogen activator(uPA)among different gastric cancer cell lines and in vestigate their relations with peritoneal metastatic potency. Methods The uPA ex pression in 4 gastric cancer cell lines(AGS,SGC7901,MKN45,MKMN28) was detected u sing ELISA and Western blot methods. uPA activity was detected simultaneously us ing uPA activity kit. The gastric cancer cells were cultured with confluent meso thelial cells in 24 well plates or Boyden chambers for different times. The adh esive cells were counted directly under a microscope. The motility and invasion of gastric cancer cells were determined by MTT assay. Results Among four gastric cancer lines,the highest expression of uPA was found in SGC7901 and the highest uPA activity in MKN45,while the lowest expression and activity of uPA in AGS. C ompared with the other three lines,MKN45 had stronger adhesion than MKMN28(P< 0. 05),SGC7901(P< 0.05),and AGS(P< 0.01),but there were no significant differences in motility and invasion among MKN45,MKN28 and SGC7901. The adhesion,motility an d invasion of AGS were weaker compared with those of the other three cell lines. Conclusions The uPA expression and activity are significantly different among 4 gastric cancer cell lines,and positively correlated with their peritoneal metas tatic potency.
出处
《中华胃肠外科杂志》
CAS
2005年第3期249-251,共3页
Chinese Journal of Gastrointestinal Surgery