摘要
目的:探讨肝炎和肝硬化疾病对尿苷二磷酸葡萄糖醛酸基转移酶(UGT)表达水平的影响。方法:应用RT-PCR和Southern杂交技术,观察肝硬化和肝炎患者肝脏组织中UGT同工酶mRNA表达水平的差异,以及炎症和纤维化程度对UGT表达水平的影响。结果:与对照组比较,肝硬化组和肝炎组UGT2B15的mRNA的水平增高为210%和227%(P<0.05),UGT1A6的mRNA的水平增高为155%和166%(P>0.05)鸦纤维化对UGT2B15RNA的表达有显著的影响,与0级纤维化相比较,4、1、2级纤维化患者UGT2B15mRNA水平分别为172%、208%和243%,三级间差异有统计学意义(P<0.01);3、1、2级纤维化患者UGT2B7mRNA水平分别为192%、208%和156%,三者间差异无统计学意义(P>0.05)。炎症程度对大多数UGT同工酶的表达无明显影响。结论:肝炎和肝硬化可影响肝脏UGT同工酶的表达水平;纤维化对UGTmRNA的表达有显著的影响,炎症程度对大多数UGT同工酶的表达无明显影响。
Objective: To investigate the influence of hepatitis and cirrhosis on hepatic UGT isoforms expression. Methods: Reverse transcription and polymerase chain reaction(RT-PCR) and southern hybridization had been used to determine the mRNA levels of five UGT isoforms in hepatic tissues. Results: There was no significant correlation between human UGT mRNA levels and the plasma levels of bilirubin, ALT, ALP or GGT in patients with hepatitis or cirrhosis. Compared to control group, UGT2B15 mRNA level of cirrhosis group and hepatitis group increased by 210% and 227% respectively (P<0.05), UGT1A6mRNA level increased by 155% and 166% respectively(P>0.05). Compared to 0 cirrhotic grade patient, UGT2B15 mRNA level of 4,1,2 grade cirrhotic patients was 172%,208%and 243% respectively(P<0.01); UGT2B7 mRNA level of 3,1,2 grade cirrhotic patients was192%,208% and 156% respectively(P>0.05). Inflammation grade had no obvious affect on mRNA expression of most UGT isoforms. Conclusion: Hepatitis and cirrhosis can affect individual UGT isoform mRNA level. Degree of fibrosis has a significant affect on UGTmRNA expression; while the degree of inflammation has no obvious affect on mRNA expression of most UGT isoforms.
出处
《山东大学学报（医学版）》
CAS
北大核心
2005年第4期306-309,共4页
Journal of Shandong University:Health Sciences
