血清中6-磷酸葡萄糖异构酶抗原升高在类风湿关节炎中的意义探讨

The significance of increased level of glucose-6-phosphate isomerase in rheumatiod arthritis patients

目的评估葡萄糖-6-磷酸异构酶(glucose-6-phosphate isom erase,GPI)在类风湿关节炎(rheum atoid arthritis,R A)发病中的意义。方法用酶联免疫吸附试验(ELISA)双抗体夹心法检测100例R A病人、98例其他风湿性疾病病人和108名健康体检者的血清中的G PI浓度,RA病人还检测了抗环瓜氨酸肽(CCP)抗体、类风湿因子(rheum atoid factor,RF)和C反应蛋白(CRP)的浓度。结果RA病人中G PI的浓度为(2.4±5.0)滋g/m l,正常对照组为(0.12±0.14)滋g/m l(P<0.01),其他风湿病组为(0.12±0.08)滋g/m l(P<0.01),GPI的浓度在RA活动组和非活动组差异有统计学意义(P<0.01);R A组23%的病人浓度高于2滋g/m l。经过Logistic回归分析发现GPI的浓度与RA病人的关节肿、疼痛成正相关。结论GPI在部分R A病人,尤其在活动期的RA病人血清中异常升高,可以作为RA临床诊断和评估的实验室指标。

Objective To assess the clinical significance of glucose-6-phosphate isomerase in RA patients. Methods The level of serum GPI in 100 patients with RA, 98 patients with other rheumatic diseases and 108 normal controls were assessed by sandwich ELISA methods. The level of RF, CRP, anti-CCP antibodies were also assessed in RA patients. Results The level of GPI was higher in RA patients [(2.4±5.0) μg/ml] than that of normal control group [(0.12±0.14) μg/ml (P<0.01)] and other rheumatic diseases group [(0.12±0.08) μg/ml(P<0.01)], and there was also significant difference in the concentration of GPI between active group and inactive group of RA patients (P<0.01). The concentration of GPI was over 2 μg/ml in 23% of RA patients. We also found the level of GPI was positively associated with the number of swollen and tender joints in RA patients by logistic regression analysis. Conclusion The concentration of GPI is abnormally increased in some RA patients, especially in active RA patients. GPI may be considered as a laboratory marker for diagnosis and activity assessment of RA.