汉防己甲素抗血吸虫病小鼠肝纤维化实验研究

THE STUDY ON TETRANDRINE AGAINST MURINE SCHISTOSOMAL HEPATIC FIBROSIS

目的探讨汉防己甲素抗血吸虫病肝纤维化作用。方法采用免疫组化法、VG染色及多媒体病理图文定量分析,观察汉防己甲素治疗前后血吸虫病肝纤维化小鼠肝脏的病理改变及Ⅰ、Ⅲ型胶原蛋白含量的变化,并与未治疗组及生理盐水对照组比较。结果汉防己甲素治疗组肝内Ⅰ、Ⅲ型胶原蛋白的含量明显下降,与生理盐水对照组及未治疗组比较,差异有显著性(P<0.01)。结论汉防己甲素可降低血吸虫病小鼠肝内Ⅰ、Ⅲ型胶原蛋白的含量,从而发挥抗肝纤维化的作用。

Objective To explore the effect of tetrandrine (TTD) on liver fibrosis of mice caused by Schistosoma japonicum infection. Methods Immunohistochemical technique, VG staining technique and multimedia pathologic picture analysis system were applied to observe the pathological change and the change of content of collagen typeⅠ and type Ⅲ in mouse liver with liver fibrosis caused by schistosomiasis before and after tetrandrine treatment and compare with untreated group and normal saline group. Results The content of collagen type Ⅰ and type Ⅲ in the group of TTD was significantly reduced comparing with normal saline group and untreated group, the difference was significant (P<0.01). Conclusion Tetrandrine could reduced the content of collagen type Ⅰ and type Ⅲ in the liver fibrosis of mice caused by schistosomiasis, therely to play the role of against the liver fibrosis.