丹皮酚抑制人大肠癌细胞增殖及其与化疗药物的协同作用

Inhibitory Effect of Paeonol on the Proliferation of Human Colorectal Cancer Cell Line HT-29 and Its Synergetic Effect with Chemotherapy Agents

目的:探讨中药丹皮酚(Paeonol,Pae)对人大肠癌细胞增殖的抑制作用及其与化疗药物的协同作用。方法:应用MTT法检测不同浓度的Pae和5-氟脲嘧啶(5-FU)、丝裂霉素(MMC)、顺铂(DDP)对体外培养的人大肠癌HT-29细胞增殖的抑制作用,同时观察Pae与化疗药物的协同作用。结果:Pae可抑制HT-29细胞增殖,并且与药物浓度及作用时间呈正相依赖关系。低浓度的Pae与化疗药物协同可产生较强的抑制细胞增殖作用。结论:Pae具有直接的抗肿瘤作用,低浓度的Pae与化疗药物合用具有协同作用。

Objective: To investigate the inhibitory effect of Paeonol on the proliferation of human colorectal cancer cell line HT-29 and its synergetic effect with chemotherapy agents. Methods: The inhibitory effect of Paeonol, 5-FU, MMC, DDP with different concentration on human colorectal cancer cell line in vitro were detected by MTT assay, and the synergetic effect of Paeonol in low concentration with chemotherapy agents was observed. Results: Paeonol could inhibit the proliferation of HT-29 cells, which showed obvious concentration-effect and time-effect relationship. Pae in low concentration had synergetic effect in inhibiting the proliferation of HT-29 cells with several chemotherapy agents. Conclusion: Paeonol has a direct anti-cancer effect and can enhance the action of several chemotherapy agents markedly, which provides evidence to clinical application.