脑溢安对缺氧大鼠脑微血管内皮细胞VEGF蛋白表达的影响

Effects of naoyian serum on VEGF protein expression in cultured rat cerebral microvascular endothelial cell with hypoxia

目的:观察脑溢安血清对体外缺氧培养的大鼠脑微血管内皮细胞血管内皮生长因子(VEGF)蛋白表达的影响。方法:大鼠用脑溢安浸膏连续灌胃3d后,心脏内采血分离出血清;用新生7dSD大鼠脑内分离培养的微血管内皮细胞(RCMEC)移入厌氧培养箱造成缺氧模型;MTT法测定细胞活性;免疫细胞化学及Wersternblotting研究脑微血管内皮细胞VEGF蛋白表达。结果:缺氧18h脑溢安组OD值较对照组增高(P<0. 05)。缺氧损伤的脑微血管内皮细胞内VEGF蛋白表达增强,脑溢安血清组VEGF蛋白的表达水平高于对照组(P<0. 01)。结论:缺氧可诱导脑微血管内皮细胞VEGF蛋白的表达;脑溢安可以上调VEGF蛋白的表达,这可能是脑溢安对缺氧的脑微血管内皮细胞的保护作用机制之一。

Objective To determine the effects of naoyian (NYA) serum on the expression of vascular endothelial growth factor (VEGF) protein in cultured rat cerebral microvascular endothelial cell(RCMEC)with hypoxia. Methods NYA serum was separated from rat heart which had been filled stomach with NYA successively for 3 days.The rat cerebral microvascular endothelial cells were taken from the Sprageu-Dawley rat brain at postborn 7 days. The rat cerebral microvascular endothelial cells were incubated at anaerobic incubator to establish the hypoxia models. The vigo of RCMEC was determined by MTT. The level of expression of VEGF protein was measured by cell immunohistochemistry and Western blot. Results The OD value of NYA serum group was higher than the control groups after hypoxia for 18 hours. VEGF protein was increased by hypoxia in cerebral microvascular endothelial cells ( P <0.05). The content of VEGF protein in NYA serum containing medium was more significantly elevated than those cultured in other control media ( P <0.01). Conclusion VEGF protein was induced by hypoxia in rat cerebral microvascular endothelial cells, and NYA could upregulate the expression of VEGF protein, which may be one of the protection mechanisms for cerebral microvascular endothelial cells.