EphB4、HIF-1α在肺癌组织中的表达及其意义

Expressions of EphB4 and HIF-1α in human lung cancer and their significances

背景与目的 业已发现EphB4和HIF 1α与肿瘤的发生、发展有密切关系。本研究的目的是探讨EphB4、HIF 1α蛋白在肺癌中表达的生物学意义及相互间的关系。方法 采用免疫组织化学SP法检测54例肺癌组织和10例正常肺组织中EphB4、HIF 1α蛋白表达的情况。结果 EphB4 和HIF 1α蛋白在54例肺癌组织中的阳性表达率分别为50.0%(27/54)和42.6%(23/54),较对照组显著升高(P<0.05);肺癌中EphB4和HIF 1α的表达与肉眼类型、分级、分期有密切关系(P<0.05),而与组织学类型、年龄、性别、淋巴结转移无明显关系(P>0.05);EphB4和HIF 1α的表达呈显著正相关关系(P<0.01)。结论 EphB4 和HIF 1α在肺癌中的异常表达与肺癌的发生、进展和恶性度有关,两者存在着一定的协同作用;检测EphB4 和HIF 1α表达有助于预测肺癌预后。

Background and objective It has been proved that EphB4 and HIF-1α are closely related to the oncogenesis and development of lung cancer. The aim of this study is to investigate the biological significances of EphB4 and HIF-1α in lung cancer and their relationship with each other. Methods The expression of EphB4 and HIF-1α was detected in 54 lung cancer tissues and 10 normal lung tissues as control by immunohistochemical method. Results EphB4 and HIF-1α proteins were detectable in 50.0% and 42.6% of all 54 lung cancer tissues respectively, which were significantly higher than those of the control (P<0.05); the positive ratios and the levels of the expressions of EphB4 and HIF-1α proteins were closely related to gross types, differentiations and clinical stages (P<0.05), but not to histological classification, age, sex and lymph node metastasis (P>0.05). A highly positive correlation was observed between EphB4 and HIF-1α expression (P< 0.01 ). Conclusion Overexpression of EphB4 and HIF-1α may play an important role in the pathogenesis, progression and malignant degree of lung cancer. Detection of EphB4 and HIF-1α expression might be helpful to predict prognosis of patients with lung cancer.