猪肝脏体外不同灌流系统建立和评价

Research of Constructing and Evaluating the System of Extracorporeal Liver Perfusion in Pigs

目的:探讨体外肝脏灌流系统中的影响因素,建立稳定有效的体外肝脏灌流系统。方法:根据灌流和氧合的方式建立不同的体外猪肝脏灌流系统,随机分为3组(每组n=4)。A组单独灌流门静脉,氧合灌流液;B组同时灌流门静脉和肝动脉,共同氧合门静脉和肝动脉的灌流液;C组同时灌流门静脉和肝动脉,分别氧合门静脉和肝动脉的灌流液。观察体外肝脏灌流时间、胆汁分泌量、病理变化和血液动力学等指标。结果:A组的灌流时间明显低于B组和C组。在1,3,6h的时间点A组胆汁分泌量、血液动力学等指标和B、C组比较差异有统计学意义。在12h时间点B组和C组胆汁分泌量和血液动力学的差异也有统计学意义。结论:同时灌流门静脉和肝动脉,分别氧合门静脉和肝动脉灌流液的灌流系统更稳定,对体外肝脏功能的维持效果较好。

Objective: To study the factors which effect the system of extracorporeal liver perfusion,and to construct a steady and effective system of extracorporeal liver perfusion. Methods: Porcine livers were harvested from health pigs, according to the difference of perfusion and oxygenating blood style ,which were randomly assigned to 3 groups (n = 4).The livers in group A were performed by single portal vein perfusion, the livers in group B were performed by dual (portal vein and hepatic artery) vessel perfusion, but oxygenating blood together,the livers in group C were performed by dual (portal vein and hepatic artery) vessel perfusion and oxygenating blood separately. The functions of porcine liver in every groups were tested. Results: At 1, 3, 6 h after perfusion, the data of group A were statistically different from group B and C. At 12 h after perfusion, the data of group B were statistically different from group C. Conclusion: The system of extracorporeal liver perfusion which was performed by dual (portal vein and hepatic artery) vessel perfusion and oxygenating blood separately is more steady and effective to keep the function of extracorporeal liver.