摘要
目的以煮沸15min灭活后的大肠杆菌O157为抗原,分4次免疫产蛋鸡,获取高免卵黄。经水稀释法去脂、超滤、盐析等粗分离,DEAESephadexA-50离子交换层析纯化,得到产物IgY为电泳单点纯,且仍保持效价在1∶104以上。从分离纯化过程中的跟踪检测表明,其卵黄制备的IgY抗体产量多、效价高、特异性强,可进一步用于大肠杆菌感染的检测研究。
The antigen of E.coli O157 was heated with boiling for 15 minutes and used to immobilize chicken for 4 times, and the antibodies produced in the egg yolk were isolated and purified through the processes of ultrafiltration, salting-out and ion-exchange chromatography of DEAE-Sephadex A-50. The specific egg yolk immunoglobulin IgY produced was checked by SDS-PAGE and its titer was determined by ELISA. It was found that the titer of anti-E.coli O157 antibody produced by this method of immunization was above 1∶10 000. The egg yolk of the immunized chicken is a rich and inexpensive source of specific antibodies with large amount of specific and high titered antibodies produced, and can be used for the further studies of the E.coli infections.
出处
《中国人兽共患病杂志》
CSCD
北大核心
2005年第5期407-409,共3页
Chinese Journal of Zoonoses