一种从人子宫颈粘液新分离的抗菌多肽的分子特性分析

Characterization of a New Antibacterial Polypeptide Isolated from Human Cervical Mucus

目的 对本室从人子宫颈粘液分离纯化的1个抗菌多肽HCP- 1进行分子结构特性分析。方法 分离HCP- 1多肽,测定其氮端氨基酸序列,根据其氮端氨基酸序列密码子设计简并引物,采用3′- RACE- PCR技术扩增HCP- 1编码基因,连接到T载体进行测序。用生物软件分析测序结果。结果 以简并引物结合RACE- PCR技术成功的克隆出HCP- 1全长c DNA,BL AST分析证实其序列与HMG- 17分子相同,OMIGA软件分析发现其含有一个α-螺旋结构(疏水结构区)可能是其抗菌功能的基础。结论 HCP- 1为HMG- 17,是存在于宫颈粘液抗菌的一种抗菌多肽,其中的α-螺旋结构可能是其抗菌的结构基础。

Objective To identify a new antibactrial polypeptide HCP-1 isolated from human cervical mucus. Methods HCP-1 was isolated and purified from human cervical mucus, and N-terminal sequence of HCP-1 was determined. A degenerate primer was designed according to CodeHop methods, and an 'anchor-oliga-dT primer' was used for the synthesis of cDNA. Using the degenerate primer and anchor-primer, cDNAs were amplified by PCR.The PCR products were cloned, sequenced, and analyzed by biological software. Results N-terminat sequence of HCP-1 was PKRKAEGDAK. The full length of HCP-1 cDNA was isolated and of which the sequence was the same as HMG-17. OMIGA software analysis indicated that this molecule contained an α-helix region. Conclusion The new antibacterial polypeptide isolated from human cervical mucus is HMG-17. It may play a role in the human cervical mucus and the α-helix domain may be its antibacterial activity region.