蜂房提取物对三种口腔常驻细菌产酸影响的体外研究

An in vitro Study on Effect of Nidus Vespae Extract on the Acid Production of Three Strains of Oral Bacteria

目的 研究蜂房提取物对三种口腔常驻细菌产酸的影响。方法 选用变形链球菌ATCC2 5 175 ,血链球菌ATCC 10 5 5 6 ,粘性放线菌ATCC 192 4 6 ,测定蜂房四个化学组分的最低抑菌浓度MIC。再以低于MIC的4个浓度配制含药的TPY液体培养基,测定蜂房各化学组分对上述三种细菌产酸能力的影响,动态监测蜂房化学组分对变形链球菌产酸的影响。结果 蜂房各化学组分对上述三种细菌的生长有一定的抑制作用,并且蜂房组分NVE1、NVE3、NVE4对变形链球菌、血液链球菌、粘性放线菌产酸均有显著的抑制作用。而蜂房组分NVE2对变形链球菌和血液链球菌的产酸未见明显的抑制作用,NVE2的高浓度组能够抑制粘性放线菌的产酸,动态p H监测显示,蜂房组分NVE1能够明显抑制变形链球菌的产酸,而NVE2则无此作用。结论 蜂房各化学组分对三种口腔常驻细菌的产酸都有一定的抑制作用,其抑龋作用的物质基础及药理作用机制尚待进一步研究。

Objective To inquire into the effect of different of Nidus Vespae extract(NVE) on growth and acid production of Streptococcus mutans, Streptococcus sanguis and Actinomyces viscosus. Methods Streptococcus mutans ATCC 25175, Streptococcus sanguis ATCC 10556 and Actinomyces viscosus ATCC 19246 were chosen as the experimental bacteria. Four extracts of Nidus Vespae were prepared and then the effects of these Nidus Vespae extracts on the acid production were determined. Results All of Nidus Vespae extracts could inhibit the growth the of the three strains, and NVE1, NVE3, NVE4 could inhibit the acid production of the three strains, NVE2 could inhibit the acid production of Actinomyces viscosus. Conclusion Four extracts of Nidus Vespae could inhibit the acid production of three bacteria strains.