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17β雌二醇的神经保护作用与磷脂酰肌醇-3-激酶的活性关系 被引量：1

Relationship between neuroprotective effect of 17β-estradiol and activity of phosphatidylinositol-3-kinase
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摘要 目的:研究17β雌二醇(βE2)保护视网膜神经细胞的作用与磷脂酰肌醇3激酶(PI3K)的活性关系.方法:应用视网膜神经细胞培养、MTT测定、PI3K活性测定及Western blot分析的方法,观察βE2与视网膜神经细胞PI3K的关系.结果:10μmol/LβE2培养细胞30min后,PI3K的活性开始上升,1h达高峰,12h恢复正常.用βE2直接与细胞匀浆37℃孵育1h,PI3K活性没有改变.而且βE2也未影响PI3K在细胞中的表达.PI3K抑制剂LY294002降低了βE2削弱H2O2的毒性作用.结论:βE2能间接激活细胞中PI3K的活性,LY294002抑制了βE2保护视网膜神经细胞的作用.提示βE2的神经保护作用可能与PI3K的激活有关. AIM: To investigate the relationship between the retinal neuroprotective effect of βE2 and the activity of PI3K. METHODS: The relationship between the retinal neuroprotective effect of βE2 and activity of PI3K was investigated by methods of cultured retinal neurons, MTT assay, PI3K assay and Western blot analysis. RESULTS: PI3K activity was increased by a 30 min treatment of cultured retinal neurons with 10 μmol/L βE2 and reached a plateau at 1 h. The PI3K activity returned to control level after 12 h. The incubation of βE2 directly with a homogenate of cultured retinal neurons did not activate PI3K and the expression of PI3K did not respond to βE2 treatment. LY294002, a PI3K inhibitor, greatly inhibited the βE2 attenuated H 2O 2-induced cytotoxicity. CONCLUSION: βE2 can significantly activate PI3K in cells in an indirect way and LY294002 can significantly inhibit the retinal nueroprotection provided by βE2, suggesting that neuroprotective effects of βE2 are related to the activation of PI3K.
作者 俞小瑞 张小玲 高广道 曹伟
机构地区 西安交通大学医学院生物化学与分子生物学教研室 西安交通大学第一医院眼科 西安交通大学医学院病理生理学教研室 俄克拉荷马大学健康科学中心
出处 《第四军医大学学报》 北大核心 2005年第9期808-811,共4页 Journal of the Fourth Military Medical University
关键词 17Β-雌二醇 磷脂酰肌醇-3-激酶 视网膜 神经细胞 estradiol phosphatidylinositol-3-kinase retina neurons
分类号 Q579.13 [生物学—生物化学]
  • 相关文献

参考文献10

  • 1Picazo O, Azcoitia I, Garcia-Segura LM. Neuroprotective and neurotoxic effects of estrogens[J]. Brain Res, 2003;990(1-2):20-27.
  • 2Behl C. Oestrogen as a neuroprotective hormon[J]. Nat Rev Neurosci,2002; 3(6):433-442.
  • 3俞小瑞,高广道.17β-雌二醇对H_2O_2诱导视网膜神经细胞死亡的作用[J].西安交通大学学报（医学版）,2004,25(4):316-318. 被引量：3
  • 4Toth G,Yang H,Anguelov RA, et al .Gene Transfer of glial cell-derived neurotrophic factor and cardiotrophin-1 protects pc12 cells from injury: Involvment of the phosphatidylinositol 3-kinase and mitogen-activated protein kinase kinase pathway[J]. J Neurosci Res, 2002; 69(5): 622-632.
  • 5Rajala RV, Anderson RE. Interaction of the insulin receptor β-Subunit with phosphatidylinositol 3-kinase in bovine ROS[J]. Invest Ophthalmol Vis Sci, 2002; 42(13): 3110-3117.
  • 6Chang JY, Liu LZ. Toxicity of cholesterol oxides on cultured neuroretinal cell[J]. Curr Eye Res, 1998; 17(1): 95-103.
  • 7Kaplan DR, Whitman M, Schaffhausen B. Common elements in growth factor stimulation and oncogenic transformation: 85 kD phosphoprotein and phosphatidylinositol kinase activity[J]. Cell, 1987; 50(7):1021-1029.
  • 8Dhandapani KM, Brann DW. Protective effects of estrogen and selective estrogen receptor modulators in the brain[J]. Biol Reprod, 2002;67(5): 1379-1385.
  • 9Spear N, Estevez AG, Barbeito L, et al. Nerve growth factor protects PC12 cell against peroxynitrite-induced apoptosis via a mechanism dependent on phosphatidylinositol 3-kinase[J]. J Neurochem,1997;69(1):53-59.
  • 10Rajala RV, McClellan ME, Ash JD, et al. In vivo regulation of phosphoinositide 3-kinase in retina through light-induced tyrosine phosphorylation of the insulin receptor β-subunit[J]. J Biol Chem, 2002;277(45):43319-43326.

二级参考文献10

  • 1Berson EL. Nutrition and retinal degenerations [J]. Int Ophthalmol Clin, 2000,40(4):93-111.
  • 2Picazo O, Azcoitia I, Garcia-Segura LM. Neuroprotective and neurotoxic effects of estrogens [J]. Brain Res, 2003, 990(1-2):20-27.
  • 3Vedder H, Anthes N, Stumm G, et al. Estrogen hormones reduce lipid peroxidation in cells and tissues of the central nervous system [J]. J Neurochem, 1999, 72(6): 2531-2538
  • 4Cambiasso MJ, Carrer HF. Nongenomic mechanism mediates estradiol stimulation of axon growth in male rat hypothalamic neurons in vitro [J]. J Neurosci Res, 2001,66(3): 475-481.
  • 5Chang JY, Liu LZ. Toxicity of cholesterol oxides on cultured neuroretinal cell [J]. Curr Eye Res, 1998, 17(1): 95-103
  • 6McGinnis JF, Stepanik PL, Chen W, et al. Unique retina cell phenotypes revealed by immunological analysis of recoverin expression in rat retina cells [J]. J Neurosci Res, 1999, 55(2): 252-260.
  • 7Iwata E, Miyazaki I, Asanuma M, et al. Protective effects of nicergoline against hydrogen peroxide toxicity in rat neuronal cell line [J]. Neurosci Lett, 1998, 251(1):49-52.
  • 8Zhang L, Zhao B, Yew DT, et al. Processing of Alzheimer's amyloid precursor protein during H2O2-induced apoptosis in human neuronal cells [J]. Biochem Biophys Res Commun, 1997, 235(3):845-848.
  • 9Dhandapani KM, Brann DW. Protective effects of estrogen and selective estrogen receptor modulators in the brain [J]. Biol Reprod, 2002, 67(5):1379-1385.
  • 10Fitzpatrick JL, Mize AL, Wade CB, et al. Estrogen-mediated neuroprotection against beta-amyloid toxicity requires expression of estrogen receptor alpha or beta and activation of the MAPK pathway [J]. J Neurochem, 2002, 82(3): 674-682.

共引文献2

同被引文献16

  • 1周开锋,蒋赞利,茅祖斌,张立峰,虞海流.17β-雌二醇对大鼠脊髓损伤后神经保护作用的研究[J].中国脊柱脊髓杂志,2007,17(8):623-627. 被引量：4
  • 2Mortimer JA, Snowdon DA, Markesbery WR. Small headcircumference is associated with less education in persons at risk forAlzheimer disease in later life [ J]. Alzheimer Dis Assoc Disord,2008,22(3) :249-254.
  • 3Green PS, Gridley KE, Simpkins JW, et al. Nuclear estrogenreceptor-independent neuroprotection by estratrienes : a novelinteraction with glutathione[ J]. Neuroscience, 1998, 84(1 ) :7-10.
  • 4Behl C,Widmann M, Trapp T, et al. 17-beta estradiol protectsneurons from oxidative stress-induced cell death in vitro [ J].Biochem Biophys Res Commun, 1995 , 216 (2) :473-482.
  • 5McClean J,Nuflez JL. 17alpha-estradiol is neuroprotective in maleand female rats in a model of early brain injury [ J]. Exp Neurol,2008’ 210(1) :41-50.
  • 6Mosselman S, Polman J, Difekma R. Identification andcharacterization of a novel human estrogen receptor[ J] . FEBS Lett,1996,392(1) :49-53.
  • 7Numakawa Y , Matsumoto T, Yokomaku D, et al. 17beta-estradiolprotects cortical neurons against oxidative stress-induced cell deaththrough reduction in the activity of mitogen-activated protein kinaseand in the accumulation of intracellular calcium [ J].Endocrinology, 2007,148(2) :627-637.
  • 8Tung N , Miron A , Schnitt SJ, et al. Prevalence and predictors ofloss of wild type BRCA1 in estrogen receptor positive and negativeBRCA1 -associated breast cancers [ J]. Breast Cancer Res, 2010,12(6) :95-96.
  • 9Toran-Allerand CD , Tinnikov AA, Singh RJ, et al. 17a-estradiol;a brain-active estrogen [ J]. Endocrinology, 2005,146 ( 9 ) : 3843-3850.
  • 10Simpkins JW, Rajakumar G, Zhang YQ, et al. Estrogens mayreduce mortality and ischemic damagecaused by middle cerebralartery occlusion in the female rat[ J]. J Neurosurg, 1997,87(5 ).

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第四军医大学学报
2005年 第9期

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