摘要
目的探讨血管抑素和内皮抑素基因在喉,鳞状细胞癌发展过程中的作用及意义。方法运用RT-PCR方法检测45例喉鳞状细胞癌组织及21例声带息肉中血管抑素和内皮抑素基因的表达,并观察它们的表达水平与喉鳞状细胞癌临床病理特征的关系。结果血管抑素和内皮抑素在喉癌组织中表达阳性率分别为71.1%和88.9%,高于声带息肉中的4.8%和14.3%。血管抑素的mRNA表达与喉癌的淋巴结转移及临床分期正相关(P<0.05),与原发部位、组织学分级和T分级无明显相关(P>0.05)。内皮抑素的mRNA表达与喉癌T分级、淋巴结转移及临床分期有关(P<0.05),与原发部位和组织学分级无明显相关(P>0.05)。结论血管抑素和内皮抑素在喉癌发展过程中具有重要意义,人工上调它们的表达将可能成为喉癌生物学治疗的有效方法。
OBJECTIVE To investigate the role and sig-nificance of angiostatin and endostatin in the progress oflaryngeal cancer. METHODS Reverse transcription-poly-merase chain reaction (RT-PCR) was used to examine themRNA level of angiostatin and endostatin in 45 cases oflaryngeal cancer and 21 cases of vocal cord polyp. The re-lationships between their expressions and clinicopathologi-cal features of laryngeal cancer were studied. RESULTSThe positive expression rates of angiostatin and endostatinin laryngeal cancer were 71.1 % and 88.9 % respectivelywhich were higher than those (4.8 % and 14.3 %) in polyp.The expression of angiostatin had close positive relation-ships with nodal metastases and clinical stage(P < 0.05)while no significantly different expression of angiostatinwas found in different primary location,T grade and histo-logical grade of laryngeal cancer. The expression ofendostatin had close relationships with T grade, nodalmetastases and clinical stage(P < 0.05) while no signifi-cantly different expression of endostatin was foundin different primary location and histological gradeof laryngeal cancer (P > 0.05). CONCLUSION Angiostatinand endostatin had an important significance in the pro-gression of laryngeal cancer, upregulating their expres-sions artificially may be an effective method in the biologi-cal treatment of laryngeal cancer.
出处
《中国耳鼻咽喉头颈外科》
北大核心
2005年第4期239-241,共3页
Chinese Archives of Otolaryngology-Head and Neck Surgery
