E-cadherin和β-catenin及CD_(44)V_6与非小细胞肺癌组织预后的关系

Prognosis significance of E-cadherin, β-catenin and CD_(44)V_6 expression in NSCLC

目的探讨上皮钙黏附素(E cad herin,E cad)、β连接素(βcatenin,βcat)及CD44V6的表达与非小细胞肺癌(non small celllungcancer,NSCLC)临床病理特征及患者预后之间的关系。方法应用免疫组织化学SP法检测53例NSCLC标本中E cad、βcat及CD44V6的表达情况。结果1)E cad及βcat在NSCLC高、中分化组及低分化组间差异有统计学意义,P值分别为0.002和0.009。而E cad及βcat的表达与临床分期、组织学类型及淋巴结转移无明显相关性,P>0.05。2)CD44V6在淋巴结转移组及未转移组间有统计学意义,P=0.017;CD44V6在鳞癌中的表达率显著高于腺癌,P=0.039。CD44V6与分化程度及临床分期均不相关,P值分别为0.0381和0.983。3)NSCLC中E cad与βcat的表达呈显著性正相关(χ2=23.11,P=0.000,r=0.66)。4)E cad及βcat高表达组的平均生存时间显著高于低表达组(P值分别为0.005和0.024)。CD44V6高表达组的平均生存时间显著低于低表达组,P=0.002。单因素生存分析显示E cad、βcat、CD44V6及临床分期与NSCLC预后有关。5)用Cox风险比例模型进行多因素生存分析显示E cad与临床分期是有意义的预后指标,P值分别为0.03和0.000。结论在NSCLC中联合检测NSCLC中E cad、βcat及CD44V6等黏附分子的表达是临床指导治疗及估计预后的有意义指标。

OBJECTIVE:To investigate the relationship bet we en the expression of E-cadherin ,β-catenin ,CD_~44 V_6 and clinicopatholo gic characteristics in (non-small cell lung cancer, NSCLC), patient post-opera tive survial was also analyzed.METHODS:The expression of E-cad herin, β-catenin and CD_~44 V_6 was immunohistochemisteically measured by SP method in 53 cases of NSCLC. RESULTS: 1)The expression of E-cad and β-cat was closely correlated with differentiated degree of NSCLC,and there was no correlation between the expression of E-cad ,β-cat and tumor st age, histological type and lymph node matestasis. 2)The positive rate of CD_~ 44 V_6 in with or without lymph node matestasis group was respectively 54.55% ,22.58%.And there was a significant difference between the two groups, P=0 .017. The high level of CD_~44 V_6 expression was detected in patients with squamous cell carcinoma more frequently than in adenocarcinoms (P=0.039). Expression of CD_~44 V_6 is independent of tomour stage and degree of differ entiation, P=0.381,P=0.983. 3)The expression of E-cad and β-cat ha d a striking positive correlation (χ2=23.11, P=0.000, r=0.66). 4) The mean survival time in cases with high level of E-cad expression was signifi cantly longer than that in cases with the low level of E-cad expression cases,b ut patients who had high level staining for CD_~44 V_6 had a poor post-oper ate survial than those with low level staining, P<0.05. The results of the univariate analysis showed that CD_~44 V_6,E-cad,tumor stage had a statisti cally significant influence on survival of NSCLC. 5)The multivariate analysis u sing Cox proportional hazards model showed that E-cad and tumor stage were usef ul prognostic indicators, P=0.03,P=0.000. CONCLUSIONS: The expression of E-cad, β-cat and CD_~44 V_6 may be of prognostic val ue in patients with NSCLC. They can be used as markers of prognosis in clinical practice.[