KDR mRNA在子宫内膜癌组织中的表达及临床意义

Expression and clinical significance of KDR mRNA in endometrial carci noma

目的研究KDRmRNA的表达与子宫内膜癌发生、发展及生物学行为的关系。方法应用逆转录聚合酶链式反应(RT PCR)技术检测子宫内膜癌45例、子宫内膜不典型增生15例和正常子宫内膜15例组织中的KDRmRNA表达情况。结果KDRmRNA在子宫内膜癌、子宫内膜不典型增生和正常子宫内膜组织中的表达率分别为73.34%(33/45)、26.67%(4/15)和20.00%(3/15),相对含量分别为118.08±15.68、97.59±10.08和80.25±21.38。子宫内膜癌组的KDRmRNA相对含量高于其他两组,P值分别为0.009和0.007;不典型增生组高于正常内膜组,但经比较差异无统计学意义,P=0.431。手术病理分期Ⅲ、Ⅳ期及有淋巴结转移、低分化组的子宫内膜癌KDRmRNA相对含量高于手术病理分期Ⅰ期、无淋巴结转移和高分化组,P值分别为0.012、0.039和0.912;而与肌层浸润深度及病理类型无关,P值分别为0.889和0.912。结论KDRmRNA的过表达在子宫内膜癌的发生、发展中起重要作用,并与子宫内膜癌的生物学行为密切相关,可作为对子宫内膜癌进行治疗的靶位点。

OBJECTIVE:To investigate the relati on ship between the expression of KDR mRNA and carcinogenesis and development of th e endometrial carcinoma. METHODS:The quantitative reverse transcription-polymerase chain reaction (RT-PCR) was used to examine the level s of KDR mRNA in the following three groups: 45 cases of endometrial carcinoma, 15 cases of hyperplasia endometrium and 15 cases of normal endometrium. RESULTS:The positive percentages of KDR mRNA expression in endometr ial carcinoma, atypical hyperplasia endometrium and normal endometrium were 73. 34%,26.67% and 20.00% respectively, and there was a singnificantly difference among these groups. The relative contents of KDR mRNA were 118.08±15.68,97.5 9±10.08 and 80.25±21.38, respectively, and the level in endometrial carcino ma was significantly higher than those in normal endometrium and hyperplasia P =0.009, 0.007, but there was no statistical significance between atypical hy perplasia and normal endometrium P=0.431.Moreover, the relative contents of KDR mRNA in endometrial carcinoma for surgical pathologic staging Ⅲ,Ⅳ, poorly differentiated tissues, with lymphatic metastasis were significantly higher tha n those in surgical pathologic staging Ⅰ, well differentiated tissues, without lymphatic metastasis P=0.012,0.039 and 0.912. However, the relative cont ents of KDR mRNA did not correlate significantly with histological type and myom etrium depth of invasion ~P=0.889 and 0.912. CONCLUSIONS: KDR may play an important role in the carcinogenesis and development of endometrial carcinoma, and correlate with the biological behavior of endometrial carcinoma, and may be a target for the treatment of endometrial carcinoma. [