肝硬化大鼠胃肠道胆碱能神经分布的变化

The changes in distribution of cholinergic nerves in gastrointestinal tract in rats with cirrhosis of liver

目的研究肝硬化大鼠胃肠动力及胃窦和小肠肌间神经丛胆碱能神经的变化。方法20只Wistar大鼠随机分为肝硬化模型组和对照组,采用葡聚糖蓝2000为胃肠内标记物观察大鼠胃肠动力变化,乙酰胆碱酯酶(AchE)组织化学染色及肌间神经丛全层铺片技术观察胃窦及小肠肌间神经丛胆碱能神经的变化,并对其分布进行定量分析。结果肝硬化组大鼠胃肠动力明显减弱(P<0.01),胃肠道肌间神经丛胆碱能阳性神经元数量减少,神经纤维变细,分布较稀疏,明显低于对照组(P<0.01或P<0.05)。结论肝硬化大鼠胃肠运动功能减退与胃肠道肌间神经丛胆碱能神经损伤有关。

Objective To investigate the changes in gastrointestinal motility and cholinergic nervous system in the gastric antrum and intestine of rats with cirrhosis. Methods 20 Wistar rats were randomly divided into control group and cirrhosis model group. The changes in gastrointestinal motility of rats were assessed by Dextran blue-2000 as an indicator; the cholinergic nerves in antro-jejunal myoenteric plexus were observed with acetylcholinesterase histochemical staining and analysed with a computer. Results Compare with control group, the gastrointestinal motility of rats was markedly retarded (P<0.01), the number density of myenteric cholinergic nerves were significantly decreased in model group (P<0.01 or P<0.05). Conclusion The gastrointestinal motility of rats with cirrhosis was markedly retarded, which may be associated with damage to cholinergic positive nerves.