大鼠FasL阳性Sertoli细胞的分离与鉴定

Isolation and identification of rat FasL-expressing Sertoli cells

目的分离鉴定Sertoli细胞,并检测其FasL的表达。方法取雄性Wistar大鼠睾丸,分离Sertoli细胞。培养72h,用多种方法进行形态学观察,做透射电镜进行形态学鉴定,免疫组化检测FasL的表达情况。结果所获得的Sertoli细胞占细胞总数的90%以上,细胞培养72h后FasL仍然高表达。结论本研究分离的Sertoli细胞可以用于细胞联合移植,发挥免疫豁免作用。

Objective To isolate and identify the Sertoli′ cells, and the expression of FasL was examined immunohistochemically. Methods Testis were isolated from male Wistar rats and Sertoli′ cells were isolated. After culturing for 72 hours, Sertoli′ cells were morphologically observed by several ways and identified with transmission electron microscope. FasL was examined immunohistochemically. Results The yielded Sertoli′ cells accounted for over 90% of all the cells harvested. FasL was expressed in high level in the 72h cultured Sertoli′ cells. Conclusion Sertoli′ cells that we have isolated can be used for co-transplantation with other cells and offer immune privilege.