白细胞介素-10对内毒素诱导急性肺损伤大鼠炎症因子的影响

Influence of interleukin-10 on the cytokine in acute lung injury in rats induced by endotoxin

目的研究白细胞介素-10(IL-10)对内毒素诱导急性肺损伤(ALI)大鼠肺组织炎症因子mRNA表达的影响,探讨IL-10对急性肺损伤的保护作用。方法24只SD大鼠随机分为3组:对照组,实验组,治疗组。实验组于尾静脉注射内毒素,治疗组注射内毒素和IL10,对照组注射生理盐水。测定各组肺组织肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)和白介素-6(IL-6)mRNA表达和血浆TNF-α、IL-1β和IL-6水平,观察各组大鼠肺组织的病理改变。结果TNF-α、IL-1β和IL6mRNA的表达:实验组、治疗组高于对照组,P<0.01;实验组高于治疗组,P<0.05。血浆TNF-α、IL1-β、IL-6:实验组高于治疗组对照组,P<0.05。肺组织病理改变:治疗组明显轻于实验组。结论IL10能抑制内毒素诱导ALI大鼠肺组织中TNF-α、IL-1β和IL6mRNA的表达,降低血浆炎症因子水平,减轻肺组织的病理损害,能起到治疗ALI的作用。

Objective To study influences of IL_10 on the cytokine in acute lung injury in rats induced by endotoxin.Methods Twenty-four SD rats were divided into three groups:control group,experiment group and treatment group.LPS was injected through tail vein in experiment group,LPS and IL-10 were injected through tail vein in treament group,and normal saline was injected through tail vein in control group.Expression of TNF-α,IL-1β,IL-6 mRNA in the lung tissue and levels of plasma TNF-α,IL-1β,IL-6 in the three groups were measured.Lung tissue was also used for pathological examination.Results The mRNA expression and the plasma levels of cytokines in experiment and treatment groups were significantly higher than those in control group(P<0.01),while experiment group were significantly higher than treatment group(P<0.05).The degree of lung injury in treatment group was significantly attenuated.Conclusion IL-10 can inhibit the expression of TNF-α,IL-1β,and IL-6 mRNA in the lung tissue,and attenuate pathological renal tissue injury.IL-10 may be effective in treating ALI.