奇异变形杆菌周期性群集运动的研究

Periodic variety in swarming motility of Proteus mirabilis

目的 研究奇异变形杆菌(proteusmirabilis ,PM )迁徙生长过程中形态、数量、呼吸酶活性等指标周期性变化。方法 在培养基中加入2 ,3 ,5 -三苯基四唑氯化物(2 ,3 ,5 tetraphenyltetrazoliumchloride ,TTC)作为呼吸酶活性的指示剂观察细菌呼吸酶活性的变化。采用透射电子显微镜对细菌形态、鞭毛进行对比观察。比浊法计数PM在时间、空间上的数量的变化。结果 PM在固体培养基迁徙生长过程中出现菌体数量逐渐减少、形态由短小到细长、鞭毛由少变多、呼吸酶从有活性向无活性转变的周期性变化。结论 PM在培养基迁徙生长过程中,微观上出现菌体长度、鞭毛数量、细菌密度等周期性变化,宏观上出现水波样的环状运动,这是环境、鞭毛、细胞间信号传递和细菌密度等多因素联合作用的结果。

Objective To investigate the periodic variety at the germ form, quantity, flagellar expression and activity of breath enzyme etc during the swarming motility of Proteus mirabilis (PM). Methods The 2, 3, 5-tetraphenyltetrazolium chloride (TTC), as indictor of breath enzyme, was added to the culture medium. Electronic microscope was adopted to observe the germ form and flagellar. Quantity of PM was measured by turbidimetry. Results During the PM growth and migration at LB medium, the germ quantity reduced and form changed from short and small to long and thin, flagellar got more and breath enzyme turned into inactive. Conclusion At the LB medium, PM in growth process appeared varieties in length of germ body, flagellar quantity and germs density, etc. PM in solid medium appeared wave motility, resulting from many factors including the environment, the signal transduction in cells and the density of germ.