PD-L1及其受体PD-1在急性肾移植排斥患者肾组织中的表达及意义

Expression and significance of programmed death ligand-1 and programmed death-l in the renal tissues following acute rejection after kidney transplantation

目的 了解急性肾移植排斥患者肾组织中PD L1、PD 1的表达及PD L1与PD 1、肾小管 间质病理损害程度的关系。方法 二步法免疫组化观察正常肾和急性肾移植排斥患者肾组织PD L1、PD 1的表达,以及PD L1阳性强度与小管 间质PD 1阳性细胞数、小管 间质病理损害程度的关系。结果 急性肾移植排斥肾组织表达PD L1、PD 1较正常组增多、增强;其小管PD L1阳性强度与肾间质PD 1阳性细胞数呈正相关,与小管 间质病理损害程度呈负相关。结论 PD L1 PD 1信号通路在急性肾移植排斥肾小管 间质病理损害中起重要作用。

Objective To investigate the role of PD-L1 in the pathogenesis of acute allograft rejection (AR) and its correlation with PD-1 and the degree of tubulointerstitial lesions. Methods A total of 20 patients admitted by our hospital and Daping hospital from 1999 to 2003 were included, 12 male and 8 female, aged 18-40 years old (average 32.2±4.5). All of them were diagnosed as acute rejection by renal biopsy. Fifteen specimens from the mismatched donor kidney and the normal tissues from the resected kidney served as normal control. Immunohistochemistry was used to detect the expression of PD-L1 and PD-1 in renal tissues of AR and normal control. Results Both PD-L1 and PD-1 expression were significantly higher in AR than that in normal control. The intensity of PD-L1 positive in renal tissues of AR showed a positive correlation with PD-1 expression and a negative correlation with the degree of tubulointerstitial lesions. Conclusion Increased expression of PD-L1 and PD-1 in renal tissues of AR has an important effect on the degree of tubulointerstitial lesions.