人前列腺癌靶向超声造影剂的制备及其体外实验研究

Preparation and immunological activity in vitro of human prostatic carcinoma-targeted liposome microbubbles

目的 制备人前列腺细胞癌靶向超声造影剂,并对其进行鉴定,探讨其体外寻靶能力。方法 静电吸附法制备免疫脂质体微泡造影剂;免疫荧光染色试验证明抗体与脂质体微泡的结合,普通光镜和荧光显微下观察微泡与前列腺癌细胞的结合情况,探讨靶向脂质体微泡对癌细胞的体外寻靶能力;同时以普通微泡作为对照。结果 免疫脂质体微泡的荧光免疫染色试验为阳性;体外寻靶试验显示携带PSM (C 15 )抗体的靶向脂质体微泡能较好地粘附于癌细胞周边;而对照组未见微泡与靶细胞的结合。结论 采用静电吸附法成功制备了具有良好免疫活性的的人前列腺癌靶向脂质体造影剂,该造影剂在体外能高效特异性地与人前列腺癌细胞结合。

Objective To prepare human prostatic carcinoma-targeted ultrasound contrast agent and assess its targeted ability in vitro. Methods Human prostatic carcinoma-specific polyclonal antibody PSM(C-15) was attached to the surface of self-made liposome microbubbles by electrostatic attraction to prepare targeted microbubbles. Immunofluorescent staining assay was used to identify the combination of PSM(C-15) with liposome microbubbles and the targeted microbubbles were added to prostatic-carcinoma cells and then observed under the light and fluorescence microscope to evaluate the targeting ability of the targeted liposome microbubbles with prostatic carcinoma cells in vitro, while the common liposome microbubbles were controls. Results Targeted microbubbles were positive in immunofluorescent straining assay. In vitro study of the targeting ability showed the targeted microbubbles could actively adhere to LNCaP cell. While the control was negative. Conclusion The targeted liposome contrast agent with highly specific biological activity was prepared successfully. The contrast agent could bind to human prostatic carcinoma cells specially and effectively in vitro.