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PKA信号通路调控体外培养大鼠脑片的CRHmRNA表达的研究

Protein kinase A signal pathway regulating corticotropin-releasing hormone mRNA expression in hypothalamic slice of rats in vitro
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摘要 目的 探讨大鼠下丘脑神经元表达促肾上腺皮质激素释放激素(CRH)mRNA的蛋白激酶A (PKA)信号调控机制。方法 通过大鼠下丘脑脑片实验模型,运用Westernblot及逆转录多聚合酶链反应(RT PCR)技术,观察CRH激活下丘脑促肾上腺皮质激素释放激素Ⅰ型受体(CRH1R)后PKA信号通路的活性变化,及其与CRHmRNA表达的关系。结果 CRH可引起下丘脑脑片磷酸化PKA、磷酸化环腺苷一磷酸反应元件结合蛋白(CREB)及CRHmRNA含量明显增加,而CP 15 45 2 6、H89可显著抑制磷酸化PKA、磷酸化CREB及CRHmRNA含量的增加。结论 在下丘脑离体脑片培养条件下,CRH可通过PKA途径实现对下丘脑神经元CRHmRNA的表达的超短正反馈调节。 Objective To investigate protein kinase A (PKA) signal regulatory mechanism of corticotropin-releasing hormone (CRH) mRNA expression, after CRH stimulated neuron of hypothalamic slices in rats in vitro. Methods After CRH stimulated corticotropin-releasing hormone type 1 receptor (CRH1R) of hypothalamic slices in rats in vitro, the changes of activity of PKA signal pathway and their relationship with CRH mRNA expression were observed by reverse transcription-polymerase chain reaction (RT-PCR), Western blotting. Results CRH may cause the remarkable increase in phosphorylated PKA (P-PKA), phosphorylated cyclic adenosine monophosphate response element-binding protein (P-CREB) and CRH mRNA content in hypothalamic slices in rats. However, CP-l54526 or H89 could have significant inhibition effects on the synthesis of P-PKA, P-CREB and CRH. Conclusion PKA signal pathway in ultrashort positive feedback control of CRH secretion in hypothalamus in the stress due to severe traumas.
出处 《第三军医大学学报》 CAS CSCD 北大核心 2005年第10期964-967,共4页 Journal of Third Military Medical University
基金 国家重点基础研究发展规划资助项目 ("973"项目 ) (G19990 54 2 0 1)~~
关键词 应激反应 下丘脑脑片 促肾上腺皮质激素释放激素mRNA 促肾上腺皮质激素释放激素受体 stress hypothalamic slices in vitro corticotropin-releasing hormone mRNA corticotropin-releasing hormone receptor
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参考文献9

  • 1Lezoua lc'h F,Engert S,Berning B,et al.Corticotropin-releasing hormone-mediated neuroprotection against oxidative stress is associated with the increased release of non-amyloidogenic amyloid beta precursor protein and with the suppression of nuclear factor-kappa B[J].Mol Endocrinol,2000,14(1):147-159.
  • 2Preil J,Muller M B,Gesing A,et al.Regulation of the hypothalamic-pituitary-adrenocortical system in mice deficient for CRH receptors 1 and 2[J].Endocrinology,2001,142(11):4946-4955.
  • 3Ono N,Samson W,McDonald J,et al.Effects of intravenous and intraventricular injection of antisera directed against corticotropin-releasing factor on the secretion of anterior pituitary hormones[J].Proc Natl Acad Sci U S A,1985,82(22):7787-7790.
  • 4Imaki T,Katsumata H,Miyata M,et al.Expression of corticotropin-releasing hormone type 1 receptor in paraventricular nucleus after acute stress[J].Neuroendocrinology,2001,73(5):293-301.
  • 5Samson W K,Taylor M M,Follwell M,et al.Orexin actions in hypothalamic paraventricular nucleus:physiological consequences and cellular correlates[J].Regul Pept,2002,104(1-3):97-103.
  • 6Nordmann J J,Morris J F.Membrane retrieval at neurosecretory axon endings[J].Nature,1976,261(5562) :723-725.
  • 7Grinevich V,Ma X M,Herman J P,et al.Effect of repeated lipopolysaccharide administration on tissue cytokine expression and hypothalamic-pituitary-adrenal axis activity in rats[J].J Neuroendocrinol,2001,13(8):711-723.
  • 8Grammatopoulos D K,Randeva H S,Levine M A,et al.Urocortin,but not corticotropin-releasing hormone (CRH),activates the mitogen-activated protein kinase signal transduction pathway in human pregnant myometrium:an effect mediated via R1alpha and R2beta CRH receptor subtypes and stimulation of Gq-proteins[J].Mol Endocrinol,2000,14(12):2076-2091.
  • 9Ellis L,Gilston V,Soo C C,et al.Activation of the transcription factor NF-kappaB in the rat air pouch model of inflammation[J].Ann Rheum Dis,2000,59(4):303-307.

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