炎症因素在泡沫细胞形成中的作用及三七皂苷对其影响

Role of inflammation in and effects of saponin of Panax Notoginseng on formation of foam cells

目的 探讨炎症因素在小鼠腹腔巨噬细胞来源泡沫细胞形成中的作用及三七皂苷对其影响。方法 在传统泡沫细胞形成模型基础上添加炎症刺激因素及三七皂苷,培养后,油红O染色观察泡沫细胞的形成,检测细胞内总胆固醇的含量,取细胞培养液测定TNF α和IL 1的含量。结果 酵母多糖能够明显增加细胞内脂质的沉积,同时伴有细胞产生TNF α和IL 1的增加,三七皂苷可以明显减少由炎症因素引起的脂质沉积的增加,并同时减少炎症细胞因子的产生。结论 炎症因素可以明显促进泡沫细胞的生成,三七皂苷可通过其抗炎活性干预这一过程。

Objective To study the role of zymosan in and the effects of total saponin of Panax notoginseng (PNS) on the formation of foam cells originating from peritoneal macrophages. Methods The peritoneal macrophages of mice were cultured with zymosan, PNS and oxidized low density lipoprotein (ox-LDL) for 48 h. The shape of the foam cells was shown with the staining of red oil O dye. The content of total cholesterol (TC) in the foam cells was determined. The concentration of TNFα and IL-1 in the supernatant of the cell culture was measured. Results The TC content and the concentration of TNF-α and IL-1 were significantly higher in the zymosan group than in the PNS group. Conclusion Inflammation can accelerate the formation of foam cells through the pathway of inflammatory cytokines but PNS can reduce the formation with the inhibition of inflammation.