GSTM1、GSTT1基因多态性与中国人前列腺癌风险关系

Polymorphisms of glutathione-S-transferase genes GSTM1 and GSTT1 and prostate cancer risk in Chinese population

目的 检测谷胱甘肽巯基转移酶GSTM1、GSTT1基因多态性与中国人前列腺癌风险关系。方法 收集重庆地区前列腺癌血标本81例,5 0岁以上对照组血标本90例,从外周血提取DNA ,采用聚合酶链反应(PCR)分析GSTM1及GSTT1无效基因型分布频率。结果 GSTM1无效基因型在前列腺癌和对照组分布频率分别为5 4 3 %、44 4% ,两组间无显著统计学差异(P =0 197,OR =1 486,95 %CI为0 813～2 718) ;GSTT1无效基因型在前列腺癌和对照组中分布频率分别为5 3 1%和5 3 3 % ,两组间无显著统计学差异(P =0 974,OR =0 99,95 %CI为0 5 43～1 80 7) ;GSTM1无效基因型合并GSTT1无效基因型在两组中的分布频率为3 0 %。13 3 % ,两组间有显著差异(P =0 0 3 4,OR =2 3 14 ,95 %CI为1 0 5 4～5 0 76)。结论 GSTM1无效基因型合并GSTT1无效基因型与重庆地区前列腺癌发病明显关联。GSTM1、GSTT1无效基因型与前列腺癌发病风险间无明显相关。GSTM1、GSTT1基因多态性与前列腺癌分期、分级无关。

Objective To observe the relationship between the glutathione S-transferase genes (GSTM1 and GSTT1) and the risk of prostate cancer (PCa) in Chinese population. Methods Blood samples from 81 prostate cancer patients and 90 controls with age above 50 in Chongqing were collected. DNA was extracted from each blood sample, the frequencies of GSTM1 and GSTT1 genotypes were analyzed using polymerase chain reaction (PCR). Results The frequencies of GSTM1 null in PCa was 54.3%, 44.4% in controls, suggesting no significant difference between them (P=0.197, OR=1.486,95%CI=0.813-2.718). The frequencies of GSTT1 null in PCa was 54.3%, 44.4% in controls, suggesting no significant difference between them (P=0.974, OR=0.99, 95%CI=0.543-1.807). The combination of GSTM1 null with GSTT1 null gene type was significantly less in PCa than that of the controls (P=0.034, OR=2.314, 95%CI=1.054-5.076). Conclusion Neither the GSTM1 null genotype nor the GSTT1 null genotype was associated with prostate-cancer risk, but the combination of them both had significant relationship with prostate cancer risk. No statistically significant associations were found between polymorphism of GSTM1 or GSTT1 with disease status and Gleason grade of PCa.