半边旗提取物5F对高转移卵巢癌细胞HO-8910PM侵袭转移的影响

Effect of 5F from Pteri semipinnata L on invasion and metastasis of highly metastatic ovarian carcinoma HO-8910 PM cells in vitro

目的研究半边旗提取物5F(以下简称5F)对人高转移卵巢癌细胞HO8910PM转移相关能力的影响及其作用的机制。方法以MTT法检测5F对高转移卵巢癌细胞HO8910PM细胞增殖的影响;以细胞粘附人工重组基底膜实验检测5F对HO8910PM细胞粘附能力的影响;用Transwell小室法检测5F对HO8910PM细胞的侵袭能力和趋化运动能力的影响;Westernblot法检测5F对HO8910PM细胞NFκB(P65)和VEGF蛋白表达的影响。结果50μmol·L-1的5F作用细胞6h后能抑制HO8910PM细胞体外侵袭人工基底膜、趋化运动和粘附能力,抑制率分别为(37.57±0.62)%,(28.42±0.67)%,(46.07±4.49)%;5F能明显下调HO8910PM细胞中NFκB(P65)和VEGF蛋白的表达。结论5F能抑制高转移卵巢癌细胞HO8910PM的侵袭、运动和粘附能力。5F抗肿瘤侵袭转移的作用机制与NFκB(P65)和VEGF蛋白的表达下调有关。

Aim To investigate the inhibitory effect and its posssible mechanism of 5F from Pteris semipinnata L on the metastasis-associated ability of human highly metastatic ovarian carcinoma HO-8910PM cells in vitro.Methods MTT assay was used to examine the effect of 5F on proliferation of HO-8910PM cells after 24 hours treatment; Transwell Chamber assay was performed to determine the effect of 5F on invasion and migratory capacity of the cells; Effect on adhesion potential of HO-8910PM cells was tested by cell-Matrigel adhesion assay; The expression levels of NF-κB(P65) and VEGF proteins were assessed by Western blot analysis.Results 5F significantly inhibited invasion, migration, and adhesion capacity of HO-8910PM cells in vitro, their inhibitory rates after treatment with 50 μmol·L -1 5F for 6 h were (37.57±0.62)%, (28.42±0.67)%, (46.07±4.49)%. 5F d istinctly down-regulated the expressions of NF-κB(P65) and VEGF protein. Conclusion 5F can inhibit the migration, invasion and adhesion of HO-8910PM cells in vitro,Its possible mechanism may be involved in the reduction of the expression level of NF-κB(P65) and VEGF. 5F might be potential drugs to inhibit tumor metastasis.