MMP-14在乳腺癌中的表达及临床意义

Significance of MMP-14 Expression in Breast Cancer

目的检测膜型1基质金属蛋白酶(MMP14)在乳腺癌中的表达并探讨其临床意义。方法采用免疫组化的方法检测46例乳腺癌手术后标本中MMP14蛋白的表达,探讨MMP14表达与乳腺癌临床病理因子的关系。结果MMP14蛋白在正常乳腺组织中没有表达,在乳腺癌中表达的阳性率是52.2%。在Ⅰ期、Ⅱ期和Ⅲ期乳腺癌中表达的阳性率分别为12.5%、54.8%和85.7%(P<0.05);在T1、T2和T3三组中的阳性率分别为11.1%、59.4%和80.0%(P<0.05);在N0、N1和N2三组中的阳性率分别为27.3%、66.7%和100%(P<0.05)。MMP14蛋白的表达与VEGR呈正相关(P<0.05),但与ER、PR、cerbB2的状况无统计学差异(P>0.05)。结论MMP14的蛋白质在乳腺癌中的表达与肿瘤分期、肿瘤大小、淋巴结转移和VEGF呈正相关,有可能为判断乳腺癌浸润转移能力的一个指标。

Objective To investigate the expression and clinical significance of MMP-14 protein in human breast cancers. Methods Forty-six human breast cancer tissues were collected. MT1-MMP protein was detected by immunohistochemistry assay and their correlations with clinicopathological factors were analyzed.Results The expression of MMP-14 protein was negative in normal breast tissues and positive in 52.2% of forty-six breast cancers tissues. The positive rates were 12.5%,54.8% and 85.7% in stageⅠ, stage Ⅱ and stage Ⅲ human breast cancers, respectively (P < 0.05), and 11.1%, 59.4% and 80.0% in the group of T 1,T 2 and T 3 (P < 0.05), and 27.3%, 66.7% and 100.0% in the group of N0,N 1 and N 2 (P < 0.05). MMP-14 protein showed positive relation with VEGF (P < 0.05), but no relations with and ER,PR or c-erbB-2(P > 0.05). Conclusion MMP-14 protein in human breast cancers had positive correlation with the stage,the tumor size,lymph node metastasis and VEGF. It can be a predictor for the ability of breast cancer invasion and metatasis.