survivin反义核酸增强PANC-1细胞对5-Fu敏感性的研究

Antisense RNA of survivin Enhances the Sensitivity of PANC-1 Cell to 5-Fu

目的研究survivin反义核酸诱导胰腺癌细胞株PANC1的凋亡和增强对5Fu敏感性的影响。方法我们应用构建的survivin反义核酸真核表达载体电转染PANC1细胞,RTPCR检测survivinmRNA表达,细胞计数和MTT实验测定转染后细胞对5Fu敏感性,流式细胞仪检测细胞凋亡。结果survivin反义核酸可抑制PANC1细胞中survivinmRNA的表达,并能抑制PANC1细胞生长,提高PANC1对5Fu敏感性,流式细胞仪检测凋亡率明显增加。结论survivin反义核酸能增强5Fu诱导的PANC1细胞凋亡,联合survivin反义核酸和5Fu可以改善胰腺癌的治疗效果。

Objective To explore the apoptosis of PANC-1 cells induced by antisense RNA of survivin and the increasing sensitivity effect to 5-Fu. Methods We transferred the construction recombination antisense RNA of survivin of eukaryotic vector into PANC-1 cells by electroporation. Expression of survivin mRNA was determined by RT-PCR. Cell Counting and MTT assay were performed to evaluate the sensibility of transferred cells to 5-Fu.The rate of apoptosis was tested by FCM. Results Transfection of the antisense RNA of survivin can inhibit the expression of survivin mRNA and inhibit the growth of PANC-1 Cells. The sensitivity of PANC-1 cells to 5-Fu was enhanced after transfection . The rate of apoptosis increased after antisense RNA of survivin was transferred into PANC-1 cells. Conclusion Antisense RNA of survivin could enhance 5-Fu-induced apoptosis in PANC-1. Combination of Antisense RNA of survivin and 5-Fu could improve the treatment effect of pancreatic cancer.