bag-1、bcl-2在大肠腺瘤和大肠癌中的表达及意义

Expression and Significance of bag-1 and bcl-2 in Colorectal Adenoma and Carcinoma

目的探讨抗凋亡基因bag1、bcl2在正常大肠黏膜、大肠腺瘤和大肠癌中的表达变化及与大肠癌发生、发展和转移的关系。方法采用免疫组化SP法,分别检测20例正常大肠黏膜、35例大肠腺瘤和82例大肠癌中bag1、bcl2蛋白表达情况。结果bag1蛋白在正常肠黏膜、大肠腺瘤、大肠癌中阳性表达率分别为10%、60%、81%。bcl2蛋白在正常大肠黏膜、大肠腺瘤和大肠癌中阳性表达率分别为25%、80%、65%。大肠腺瘤和大肠癌中bag1、bcl2蛋白表达阳性率均显著高于正常大肠黏膜(P<0.05)。bag1蛋白表达与大肠癌分化程度、Duke's分期及淋巴结转移有关(P<0.05)。而bcl2蛋白表达与性别、年龄、癌肿分化程度、Duke's分期及淋巴结转移无关(P>0.05)。结论bag1、bcl2蛋白高表达对大肠癌的发生均起重要作用,而bag1表达水平与大肠癌的恶性程度有关,可作为预测大肠癌浸润转移潜能的新的生物学指标。

Objective To investigate the expression of anti-apoptotic gene bag-1 and bcl-2 and their clinical significances in colorectal carcinoma .Methods The expressions of bag-1 and bcl-2 in 20 normal colorectal mucosa、35 colorectal adenoma and 82 colorectal carcinoma were examined with S-P immunohistochemical staining. Results The positive rates of bag-1 in normal colorectal mucosa、colorectal adenoma and carcinoma were10%、60%、81% respectively. The positive rates of bcl-2 in colorectal mucosa、adenoma and carcinoma were 25%、80%、65% respectively. The positive rates of bag-1 and bcl-2 expression in colorectal adenoma and carcinoma were significantly higher than that in normal colorectal mucosa(P< 0.05) .A significant correlation was found between bag-1 expression in colorectal carcinoma and their clinicopathological featers such as differentiation degree、 Duke's stage and lymph node metastasis (P< 0.05) . The expressions of bcl-2 were not related to the age and sex of patients、 differentiation degree、Duke's stage and lymph node metastasis (P> 0.05) . Conclusion bag-1 and bcl-2 overexpression might play an important role in the occurence of colorectal carcinoma.The expression levels of bag-1 protein is related to the malignant degree of colorectal carcinoma and may be a new biomarker in predicting the potent invasion and the metastasis of colorectal carcinoma.