卵巢癌患者CA125表达和免疫状态关系研究

A Study on the Relation between CA125 and Immunology State of Patients with Ovarian Cancer

目的探讨卵巢癌患者血清CA125和机体免疫功能的关系及其临床意义。方法采用流式细胞术检测患者外周血细胞CD3+、CD4+、NK+(CD3-/CD(16+56)+)浓度细胞的百分率,采用酶联免疫法检测患者血清CA125的浓度。结果卵巢癌患者血清CA125浓度显著高于正常对照组(P<0.01):患者CD3+、CD4+、和NK+细胞均显著低于正常对照组(P<0.05或P<0.01)。随着患者血清CA125浓度的增加,CD3+、CD4+、NK+细胞均逐渐降低,并呈显著的负相关(相关系数分别为r1=-0.959,P<0.01;r2=-0.923,P<0.05;r3=-0.912,P<0.05)。随着临床分期的增加,CA125浓度逐渐增加,CD3+、CD4+和NK+细胞则逐渐降低;伴肿瘤转移者的CA125浓度显著高于未转移者(P<0.01),而CD3+、CD4+和NK+细胞却显著低于未转移者(P<0.05)。结论卵巢癌能引起患者血清CA125表达率明显上升,而机体免疫功能显著下降。CA125的表达与患者免疫状态之间以及它们与临床生物学行为之间都有一定关系。

Objective To investigate the relation and clinical significance between CA125 expression level and immunological state of patients with ovarian cancer. Methods The concentration of serum CA125 and the percentage of CD 3 +、CD 4 +and NK + cells (CD 3 -/CD (16-56) +) were measured in peripheral blood cells from 45 patients with ovarian cancer by ELISA and flow cytometry.Results The concentration of serum CA125 in patients with ovarian cancer was significantly higher than that of normal control(P < 0.01).The percentage of CD 3 +、CD 4 + and NK + cells of patients with ovarian cancer were significantly lower than those of normal control(P < 0.01 or P < 0.05) .The correlation analysis showed that the concentration of serum CA125 were negatively correlated to CD 3 +、CD 4 + and NK + cells percentage (r 1= -0.959, P < 0.01;r 2= -0.932, P < 0.05;r 3= -0.912,P < 0.05).The gradually increasing of serum CA125 concentration and the gradually decreasing of CD 3 +、CD 4 + ,and NK + cells percentage were with increasing of clinical stages on patients with ovarian cancer .Serum CA125 concentration of patients with metastasis was higher than that of patients with non-metastasis (P < 0.01).The percentage of CD 3 +、CD 4 + and NK + cells of patients with metastasis were lower than those of patients with non-metastasis (P < 0.05).Conclusion Ovarian cancer may induce significant increasing of the concentration of serum CA125 and may induce significant decreasing of percentage of CD 3, CD 4 and NK cells.There is a much closed relationship between the concentration of serum CA125 and the immunological state of patients as well as the clinical biological behaviours of patients with ovarian cancer.