地塞米松对A系小鼠胚腭突间充质细胞增殖代谢的影响

Effects of dexamethasone on the proliferation and metabolism of A/J mouse embryonic palate mesenchymal cells

目的 研究不同剂量地塞米松(DEX)对A系小鼠胚腭突间充质细胞增殖和DNA、蛋白质及PGE2 合成的影响。方法 实验分为9组,每组设平行4孔(瓶) ,每组分别加入DEX使其终浓度为0 .0 5ng/ml,0 .1ng/ml,0 .5ng/ml,1ng/ml,5ng/ml,1 0ng/ml,5 0ng/ml,1 0 0ng/ml,1 0 0 0ng/ml,每组均设空白对照,在实验后第1、3、5天分别检测细胞的OD值及PGE2 的含量,并在第3天时检测DNA、蛋白质的含量。结果 DEX可显著增加腭突间充质细胞的OD值,促进DNA及蛋白质合成,在含量0 .5ng/ml时DEX的促进生长作用最强。在培养早期DEX轻度降低腭突间充质细胞PGE2 的合成,而在培养后期则表现为显著抑制作用。结论 DEX显著促进腭突间充质细胞的增殖代谢,可能干扰腭突间充质细胞的正常生长代谢而影响了腭发育。

Objective To study the effects of dexamethasone(DEX) with different concentration on the synthesis of DNA,protein and PGE2 of A/J Mouse Embryonic Palate Mesenchymal(EPM) Cells.Methods Nine experiment groups, each with 4 pores(or bottles),were divided with 9 gradient concentration of VEGF( 0.05, 0.1, 0.5,1,5,10,50,100, 1000ng/ml), then the DNA,protein and PGE2 of A/J EPM Cells were measured after 1 day,3 days,5 days.Results DEX stimulated the synthesis of DNA,protein of EPM Cells,and augmented proliferation index (PIX). Its effects were very obvious in proliferation and Metabolism of A/J EPM Cells when the concentration was 0.5ng/ml.In early stage of culture,DEX inhibits PGE2 synthesis of A/J EPM Cells,but it could suppress prominently the synthesis of PGE2 at anaphase of culture.Conclusion DEX can stimulate proliferation and metabolism of EPM Cells,and may disturb the development of palatal shelves.